DABS-Cl柱前衍生-HPLC测定稻米中γ-氨基丁酸方法优化  

Method Optimization of Measuring γ-Aminobutyric Acid Contents in Rice by HPLC Coupled with Dabsyl Chloride Precolumn Derivatization

作　　者：邵金良[1] 杨东顺[1] 樊建麟[1] 刘兴勇[1] 杜丽娟[1] 王丽[1] 汪禄祥[1] 刘宏程[1] 

机构地区：[1]云南省农业科学院质量标准与检测技术研究所,云南昆明650223

出　　处：《作物杂志》2016年第1期129-134,共6页Crops

基　　金：农业部2013年行业标准制定和修订项目<稻米中γ-氨基丁酸的测定>;云南省科技惠民专项(农业)重点项目(2014RA054);云南省科技创新平台建设计划(公共科技服务)项目(2014DA001)

摘　　要：建立4-二甲胺基苯基偶氮苯磺酰氯柱前衍生一高效液相色谱测定稻米γ-氨基丁酸（GABA）含量的方法。样品采用80％的乙醇溶液提取后，1．0mL样液依次加入0．20mL碳酸氢钠和0．40mLDABS-Cl在70℃下衍生20min，利用C18色谱柱，乙腈-三水合乙酸钠溶液作为流动相在436nm波长下测定GABA含量。结果显示：GABA在糙米和精米的添加回收率大于87．60％，相对标准偏差小于9．07％；GABA在1．0～100．0mg／L内具有良好的线性关系，相关系数为r=0．9995（n=3）。用本方法测定了14种糙米和精米样品的GABA，其含量为6．38～108．7mg／kg，湖南精米和糙米中的GABA含量最高。A method of measuring γ-aminobutyric acid （GABA） content in rice was established by high performance liquid chromatography coupled with dabsyl chloride pre-column derivatization. Using 80% ethanol as extracting solvent, and then 1.0mL extracted fluid pre-column derivatization with 0.20mL sodium bicarbonate, 0.40mL dabsyl chloride at 70℃ and 20min, chromatographic analysis was performed on C18 column eluted with an acetonitrile- sodium acetate trihydrate and ultraviolet detector at 436nm. The results showed that the fortified recovery of GABA was more than 87.60% and the standard deviation was less than 9.07%. A good linear range from 1.0mg/L to 100.0mg/L concentration of GABA with correlation of 0.9995 （n=3） was obtained. GABA content in 14 rice and brown rice （6.38- 108.7mg/kg） were determined by the method, and the content of GABA in Hunan rice was the highest.

关 键 词：4-二甲胺基苯基偶氮苯磺酰氯 柱前衍生 高效液相色谱法 Γ-氨基丁酸 稻米 

分 类 号：S511[农业科学—作物学]