不同提取方法对武当山地区二翅六道木叶果胶抗氧化性的影响  

Influence of Different Extracted Methods on Anti-oxidative Activity of Pectin Extracting from Abelia Macrotera Leaves of Wudang Mountain Area

作　　者：李洪亮[1,2] 杨念[1,2] 向龙超 曹凤军[1] 刘明[1,2] 汪选斌[1,2] 冯奕斌[3] 

机构地区：[1]湖北医药学院附属人民医院肿瘤中心中药药理实验室,湖北十堰442000 [2]湖北医药学院武当特色中药研究湖北省重点实验室,湖北十堰442000 [3]香港大学中医药学院

出　　处：《湖北医药学院学报》2015年第6期552-555,共4页Journal of Hubei University of Medicine

基　　金：湖北省教育厅科学技术研究项目(B2014046);湖北省高等学校优秀中青年科技创新团队计划(T201510);湖北省自然基金面上项目(2014CFB642);湖北医药学院优秀中青年团队项目(2014CXG03);湖北省学科建设项目(2014XKJSXJ18);湖北省2011计划项目(2011JH-2014CXTT09);十堰市科技局引导性项目(15Y36);武当特色中药研究湖北省重点实验室(湖北医药学院)开放课题项目(WDCM001)

摘　　要：目的:研究不同提取方法对武当山地区二翅六道木叶果胶抗氧化能力的影响。方法:采用酶提取、水提取及超声波辅助提取三种方法,用2,2-联苯基-1-苦基肼基自由基(DPPH)清除率,亚铁还原能力(FRAP)实验两种方法测定其抗氧化活性,并分析不同提取方法对其抗氧化性能力的影响。结果:在45℃时,用酶法提取60 min的果胶DPPH自由基清除率和FRAP抗氧化能力最强,DPPH自由基清除率达到(63.54±5.35)%,该方法明显优于同等条件下另外两种提取方法。结论:酶提取60 min的方法更适合于武当山地区二翅六道木叶中果胶的提取。Objective To compare the antioxidative effects of pectin extracted from Abelia macrotera in Wudang Mountain area with three different methods.Methods Enzyme extracting（ EE）,water extracting（ WE） and ultrasound- assisted extracting（ UAE） methods were conducted for extracting pectin from Abelia macrotera leaves,respectively.Then the indexes of 2,2-Diphenyl-1-picrylhydrazyl（ DPPH） clearance and ferric-reducing antioxidant power（ FRAP） were used to evaluate the antioxidative activity of pectin,and to analyze the influence of different extracting methods. Results Pectin that extracting with EE method at 45 ℃ for 60 min had the highest DPPH clearance and the strongest antioxidant capacity,the clearance of DPPH was high to（ 63.54±5.35） %.The EE method was obviously superior to the other two method at the same terms.Conclusion The enzyme extracting method with 60 min may be more suitable for extracting pectin from Abelia Macroterathe leaves in Wudang Mountain area.

关 键 词：二翅六道木 果胶 提取工艺 抗氧化性 

分 类 号：R284.2[医药卫生—中药学]