芒草木质素含量对里氏木霉产木质纤维素酶的影响  

Effects of lignin on Trichoderma reesei producing cellulase and xylanase in Miscanthus substrates

作　　者：康永波[1,2,3] 李傲[1,2,3] 裴岩杰 涂媛苑 周诗光[1,2,3] 魏小洋[1,2,3] 李庆[1,2,4] 郝勃[1,2,5] 夏涛[1,2,5] 彭良才[1,2,3] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070 [2]华中农业大学生物质与生物能源研究中心,湖北武汉430070 [3]华中农业大学植物科学技术学院,湖北武汉430070 [4]华中农业大学理学院,湖北武汉430070 [5]华中农业大学生命科学技术学院,湖北武汉430070

出　　处：《生物技术》2015年第6期604-612,共9页Biotechnology

基　　金：教育部;国家外国专家局高等学校学科创新引智项目(No.B08032);华中农业大学长江学者推进项目(No.52204-07022);中央高校基本科研业务费专项基金(No.2662015PY019)资助

摘　　要：[目的]研究芒草木质素含量对里氏木霉产木质纤维素酶的影响。[方法]选择了2组木质素含量差异显著（p〈0．01，n=3）、纤维素和半纤维素含量相近的芒草材料作底物来培养3株里氏木霉（野生型QM6a、突变型QM9414和RutC30），对诱导产生的木质纤维素酶的酶活和降解木质纤维素的效率进行分析。[结果]在第1组中，Mfl40木质素含量比Msa02低36％（P〈0．01，／Z=3），其诱导3株菌生产的木质纤维素酶体积比酶活高于Msa02组1．2～1．8倍（P〈0．05或者P〈0．01，n=3）；在第Ⅱ组中，Mfl37木质素含量比Mlu13低48％（P〈0．01，n=3），其生产的木质纤维素酶体积比酶活高于Mlu13材料1．2—3．3倍（p〈0．05或者P〈0．01，n=3）。[结论]芒草细胞壁中木质素含量对里氏木霉产木质纤维素酶量有显著负影响。[ Objective ] The influence of lignin on Trichoderma reesei producing cellulase and xylanase in Miscanthus sub- strates. [ Methods] Two pairs of Miscanthus were selected with distinct lignin levels, and their biomass residues were incubated with three Trichoderma reesei strains （Wild type： QM6a; Mutants： QM9414 and RutC30） for cellulase and xylanase produc- tion. [ Results] The Mfl40 sample at Pair I showed a lower lignin level than that of Msa02 by 36% at p 〈 0.01 level （ n = 3 ）, but it resulted in three Trichoderma reesei strains producing much higher holocellulase （ cellulase and xylanase） activities 1.2 - 1.8 at p 〈0.05 and p 〈0.01 levels（n --3） respectively,compared with the Msa02. Meanwhile,the Mf137 in the Pair II ,also exhibited much higher holocellulase activities by 1.2 - 3.3 at p 〈 0.05 and p 〈 0.01 levels（ n = 3 ） respectively than those of Mlul3 sample, due to its relatively lower lignin level by 48%. [ Conclusion] The cellulase and xylanase productions released by three Trichoderma reesei strains were negatively affected by lignin levels of Miscanthus.

关 键 词：里氏木霉 芒草 木质素 纤维素酶 木聚糖酶 酶解 

分 类 号：Q939.97[生物学—微生物学]