肾茶多酚提取工艺及其抗氧化活性研究  

Extraction and Antioxidant Activity of Polyphenols from Clerodendranthus spicatus

作　　者：李晓花[1] 陈蕾西 牛迎凤[1] 刘颖颖[1] 张丽霞[1] 

机构地区：[1]西双版纳州傣药南药重点实验室中国医学科学院北京协和医学院药用植物研究所云南分所,景洪666100 [2]中国药科大学生命科学与技术学院,南京210009

出　　处：《天然产物研究与开发》2016年第2期257-261,共5页Natural Product Research and Development

基　　金：中国医学科学院药用植物研究所中央级公益性科研院所基本科研业务专项基金(YNYZ-13-07)

摘　　要：采用回流提取法,进行单因素试验和L9（34）正交试验,确定肾茶多酚最佳提取工艺;并通过测定1,1-二联苯基-2-苦肼自由基（DPPH·）和羟自由基（·OH）清除率评价其抗氧化活性。得到的肾茶多酚的优化提取工艺为液料比10∶1（m L/g）,50%乙醇回流3次,每次提取2 h,肾茶多酚提取率为3.60%,优选的提取工艺稳定可靠,重现性好。体外抗氧化活性实验结果显示：肾茶多酚的抗氧化能力随质量浓度的增大而增强,在1~10μg/m L浓度范围内和100~1250μg/m L浓度范围内,其清除DPPH自由基和羟基自由基的能力略强于抗坏血酸,两者的对DPPH自由基半数抑制浓度分别为5.78和6.31μg/m L;对羟基自由基的半数抑制浓度为851.1和940.1μg/m L。Reflux extraction method was used to extract the polyphenols from Clerodendranthus spicatus in this study. Single factor experiments and the L9 （3^4） orthogonal experiment was applied to optimize the extraction conditions. In addition,the antioxidant activities of the extract were evaluated by DPPH · and · OH scavenging assays. The optimal ex- traction conditions for extracting polyphenols were as follows： liquid/solid ratio of 10：1 mL/g, ethanol concentration of 50%, 2 h per time, and extract 3 times. Under the optimized conditions, the extraction yield of total polyphenol was up to 3.60%. In vitro antioxidant activity of total polyphenols increased with the increasing of concentration,the scavenging abilities against DPPH ·and · OH were stronger than vitamin C within the range of 1-10 μg/mL and within the range of 100-1250 μg/mL,respectively. The ICso values were 5.78 and 6.31 μg/mL toward DPPH · ,and 851.1 and 940.1 μg/ mL toward · OH, respectively.

关 键 词：肾茶 多酚 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]