不同溶剂绞股蓝提取物清除自由基性能  

Ability of Free Radical Scavenging by Different Gynostemma Extracts

作　　者：王蕾[1] 甘美娇 刘泽宏[1] 王依楠[1] 朱妙琴[1] 

机构地区：[1]浙江外国语学院科技学院,浙江杭州310012

出　　处：《广州化工》2016年第4期61-62,共2页GuangZhou Chemical Industry

摘　　要：分别以80℃水和25℃50%的乙醇溶液为提取溶剂,固液比1∶30,以普通震荡法提取绞股蓝中黄酮类(包括儿茶素、原花青素)物质,紫外显色法测定两种提取液中黄酮类物质含量。结果表明:提取液中黄酮类物质含量分别为18.0 mg/g和6.00 mg/g,提取液温度对绞股蓝中黄酮类物质的提取效果影响较大。80℃水提取液比25℃50%的乙醇提取液清除DPPH自由基的能力更强;清除自由基能力与提取物中黄酮类物质含量并不完全呈正比。To optimize the extracting total flavonoids,80 ℃ water and 25 ℃ 50% ethanol were used to extract the flavanolin Gynostemma,include catechin and polyprocyanidin. The contents of flavanols were determined in two extracts.The contents of flavanol were 18. 0 mg / g by 80 ℃ water and 6. 00 mg / g by 50% ethanol. The 80 ℃ water was batter than50% ethanol on extracting the contents of flavanol in Gynostemma. The 80 ℃ water extracts were more batter on scavenging of DPPH（ 1,1- Diphenyl- 2- picrylhydrazyl） than 50% ethanol. It was no effective on free radical scavenging activity to contents of flavanols.

关 键 词：绞股蓝 黄酮类物质 清除自由基 

分 类 号：TQ914.1[化学工程]