饲料发酵耦合黄曲霉毒素B_1的生物脱除方法研究  

Simultaneous Feed Fermentation and Biodegradation of Afltoxin B_1 with Candida utilis

作　　者：李加友[1] 褚云峰 陆筑凤[1] 苗圣威 李俊峰[1] 杨青 

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [2]嘉兴市南湖饲料有限公司,浙江嘉兴314001

出　　处：《中国畜牧杂志》2016年第4期59-62,共4页Chinese Journal of Animal Science

基　　金：嘉兴市科技局资助项目(2013BY26001);嘉兴市南湖区科技局资助项目(2012QN39)

摘　　要：饲料原料在贮运和加工过程中常被黄曲霉毒素污染，造成严重的经济损失和食品安全隐患。利用单因素比较法确定产朊假丝酵母（Candida utilis）JXU2066在饲料发酵过程中的脱毒控制方法。结果表明：在被黄曲霉毒素污染的玉米中添加适量的麸皮和尿素，加水至体系含水量为45％，调节pH为4—5，以固体基质含量8％的比例接入茵龄为72h的产朊假丝酵母JXU2066，并且根据堆温翻动2次，30d后的发酵饲料中黄曲霉毒素含量由230μg／kg降为8．7μg／kg，粗蛋白含量为15．6％，发酵饲料色泽金黄，气味芳香。利用产朊假丝酵母在固态发酵条件下实现黄曲霉毒素的生物脱除，操作简单，脱毒率高，具有良好的应用价值。Feedstuffs in the storage and shipping processes are often contaminative by aflatoxins, which causes seriously financial loss and safety loophole. Single-factor comparison is used to optimize the solid fermentation process for aflatoxin removing and feed quality developing. Results showed that aflatoxin B1 contaminated in corm was decreased from 230 μg/kg to 8.7μg/kg and protein content is up to 15.6% after 30 d fermentation with the following conditions： corn powder mixed with appropriate bran and urea, keeping water content 45%, the pH adjusted to 4-5, with 8% of 72 h Candida JXU2066 as inoculant, turning 2 times on the base of heap temperature in fermentation process. Fermented feed was golden with fragrant smell. It is a potential method to remove aflatoxin in feed with solid fermentation for its high detoxication rate and simple operation.

关 键 词：黄曲霉毒素 发酵饲料 产朊假丝酵母 生物脱毒 

分 类 号：S816.32[农业科学—饲料科学]