海地瓜岩藻聚糖硫酸酯对胰岛素抵抗小鼠肝脏炎症反应的影响  

ANTI-INFLAMMATORY EFFECTS OF FUCOIDAN FROM ACAUDINA MOLPADIOIDEA IN THE LIVER OF INSULIN RESISTANT MICE

作　　者：胡世伟[1,2] 王静凤[2] 李世杰[1] 姜维[1,2] 宋文东[1] 

机构地区：[1]浙江海洋学院创新应用研究院,舟山316022 [2]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《营养学报》2016年第1期24-29,35,共7页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.31371876;31471648);浙江海洋学院科研启动经费(No.Q1442;Q1443)

摘　　要：目的研究海地瓜岩藻聚糖硫酸酯(fucoidan form Acaudina molpadioidea,Am-FUC)对胰岛素抵抗小鼠肝脏炎症反应的改善作用及机制。方法采用饲喂高脂高糖饲料法建立胰岛素抵抗小鼠模型,给予80mg/kg·bw的Am-FUC 19w。实验结束后,检测模型小鼠血清促炎症因子和抗炎症因子浓度和血清和肝脏FFA水平,用实时荧光定量PCR方法检测Am-FUC对肝脏组织炎症状因子基因m RNA表达,Western blotting法检测Am-FUC对胰岛素抵抗小鼠肝脏组织JNK和IKKβ/NFκB炎症通路的影响。结果 Am-FUC能显著地降低胰岛素抵抗小鼠血清促炎症因子浓度并增加抑炎症因子浓度(P<0.01),降低血清和肝脏FFA浓度(P<0.01),抑制抑炎症因子基因m RNA表达水平并促进抑炎症因子基因m RNA表达水平(P<0.05,P<0.01),抑制肝脏组织JNK1和IKKβ蛋白磷酸化(P<0.05,P<0.01)、增加细胞质中NFκB蛋白表达(P<0.01),抑制细胞核中NFκB蛋白表达(P<0.01)。结论 Am-FUC能通过抑制JNK和IKKβ/NFκB炎症通路起到改善胰岛素抵抗小鼠肝脏炎症反应的作用。Objective To investigate the effects of fucoidan from Acaudina molpadioidea（Am-FUC） on alleviating inflammatory response in insulin resistant mice. Methods The insulin resistance mouse model was established by feeding a high-fat a high-sucrose diet. The diabetic mice were given Am-FUC at 80 mg·（kg·body weight）-1 for 19 weeks. Then, serum pro-inflammatory and anti-inflammatory cytokines concentrations, the free fatty acids levels in serum and liver were determined. Quantitative real time PCR was used to assay m RNA expression of inflammatory cytokine genes. Western blotting was used to measure proteins expression levels in JNK and IKKβ/NFκB inflammatory pathways in the liver of insulin resistant mice. Results Am-FUC could significantly reduce pro-inflammatory cytokine secretion and increase anti-inflammatory cytokines production, decrease serum and hepatic FFA levels, inhibit m RNA expression of pro-inflammatory cytokines and promote anti-inflammatory cytokines m RNA cxpression level, inhibit hepatic phosphorylated proteins of JNK1 and IKKβ, increase cytoplasmic NFκB protein and decrease nuclear NFκB protein. Conclusion Am-FUC alleviates inflammatory response via inhibition of JNK and IKKβ/NFκB pathways in the liver of insulin resistant mice.

关 键 词：海地瓜 岩藻聚糖硫酸酯 炎症因子 游离脂肪酸 氨基末端激酶 核转录因子ΚB 

分 类 号：R151[医药卫生—营养与食品卫生学]