细胞絮凝及硫酸锌对酿酒酵母乙酸胁迫耐性的影响  

Effects of cell flocculation and zinc sulfate addition on acetic acid stress tolerance of Saccharomyces cerevisiae

作　　者：程诚[1] 赵心清[2] 白凤武[1,2] 

机构地区：[1]大连理工大学生命科学与技术学院,大连116024 [2]上海交通大学生命科学技术学院,上海200240

出　　处：《应用与环境生物学报》2016年第1期116-119,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家高技术研究发展计划(863计划)项目(2012AA021205;2012AA101805);国家自然科学基金项目(21376043)资助~~

摘　　要：酿酒酵母细胞絮凝和外源添加锌离子对其环境胁迫耐受性都具有促进作用,为了解细胞絮凝形态对锌促进乙醇发酵的影响,比较硫酸锌添加对絮凝酿酒酵母SP SC01及其絮凝基因失活突变体SPSC01 FLO1Δ在乙酸胁迫条件下乙醇发酵的影响.结果显示,与野生型絮凝酵母相比,添加锌可更明显改善SPSC01 FLO1Δ在乙酸中的生长和发酵,在10 g L^(-1)乙酸存在的情况下,SPSC01在70 h消耗100 g L^(-1)葡萄糖,锌离子添加后可使发酵终点提前10 h,而SPSC01FLO1Δ在锌离子添加后发酵时间为48 h,可将发酵时间显著缩短138 h.这些结果表明,锌在酿酒酵母细胞缺少絮凝保护的条件下更能有效发挥作用,同时甘油、琥珀酸的增加在絮凝基因敲除突变体中更加明显.本文研究结果可为进一步利用絮凝及锌响应调控基因提高酿酒酵母的环境胁迫耐受性,提高燃料乙醇的生产效率奠定基础.Both flocculation and zinc supplementation are beneficial for protecting Saccharomyces cerevisiae against stressful conditions,however,the effect of cell flocculation on the enhancement of ethanol production by zinc addition is still unclear.This study compared the ethanol production ability of the flocculating yeast strain SPSC01 and its FLO1△ mutant in the presence of acetic acid with the supplementation of zinc sulfate.It was found that zinc sulfate supplementation exerted more significant effect on the improvement of cell growth and ethanol fermentation abilities of the FLO1 disruption mutant compared to SPSC01.Under the stress of 10 g L^（-1） acetic acid,the fermentation time for SPSC01 was 70 h without zinc addition,but shortened by 10 h with zinc supplementation;the FLO1△ mutant could consume all the glucose in 48 h with zinc sulfate supplementation,138 h in advance than that without zinc sulfate addition.The results indicated that the protection ability of zinc to yeast cells was more obvious in yeast cells lacking flocculation protection,suggesting different mechanisms underlying improved stress tolerance by cell flocculation and zinc addition.These results provide basis for further improvement of stress tolerance of yeast cells against unfavorable environmental conditions,and benefits strain development using cell flocculation and zinc-responsive genes for efficient cellulosic ethanol production.

关 键 词：自絮凝酵母SPSC01 絮凝基因FLO_1 乙酸耐受性 锌添加 乙醇发酵 

分 类 号：TS261.11[轻工技术与工程—发酵工程]