EPHB6缺失突变del915-917对非小细胞肺癌体内转移的影响  

Del915-917,the deletion mutation of the EPHB6,promotes in vivo metastasis of non-small cell lung cancer in NOD/SCID mice

作　　者：于军[1] 铁茹[1] 张学策[1] 常盼[1] 赵鸽[2] 陈宝莹[3] 

机构地区：[1]西安医学院第二附属医院中心实验室,西安710038 [2]西安交通大学第一附属医院麻醉科 [3]第四军医大学唐都医院放射科

出　　处：《陕西医学杂志》2016年第2期133-135,共3页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(30801385);卫生部肿瘤个体化治疗分子诊断专项课题(W2013FZ20);陕西省社会发展攻关课题(2014K11-01-01-07);陕西省教育厅基础研究专项课题(14JK1616)

摘　　要：目的:探讨EPHB6缺失突变del915-917对非小细胞肺癌(NSCLC)体内转移的作用。方法:定点突变EPHB6的表达载体pcDNA4-EPHB6-wt获得突变载体pcDNA4-EPHB6-del915-917,转染肺腺癌细胞A549,进而将细胞接种于NOD/SCID小鼠体内,通过转移灶计数、HE染色评价转移情况。结果:表达EPHB6-del915-917的NSCLC细胞体内转移显著强于表达野生型EPHB6-wt的细胞。结论:EPHB6的缺失突变del915-917促发NSCLC体内转移。Objective：To clarify the effects of del915-917,a deletion mutation of EPHB6,on the in vivo metastasis of NSCLC（non-small cell lung cancer）in NOD/SCID mice.Methods：Site-directed mutagenesis was performed from pcDNA4-EPHB6-wt to pcDNA4-EPHB6-del915-917,which was transfected into A549 lung adenocarcinoma cells.The cells were then injected into NOD/SCID mice.The metastatic status were evaluated by metastasis counting and HE staining.Results：Cells expressing EPHB6-del915-917 demonstrated significant higher levels of metastasis,compared with cells expressing EPHB6-WT.Conclusion：The deletion mutation del915-917 increased the metastatic capability of NSCLC cells in NOD/SCID mice.

关 键 词：癌 非小细胞肺 肿瘤转移 移码突变 @EPHB6 

分 类 号：R734.1[医药卫生—肿瘤]