草鱼鱼鳞胶原蛋白的提取研究  

Extraction of collagen from grass carp scales

作　　者：李卫林[1,2] 洪洪[2] 汤克勇[1] 

机构地区：[1]郑州大学材料科学与工程学院,河南郑州450001 [2]武夷学院生态与资源工程学院,福建武夷山354300

出　　处：《中国皮革》2016年第3期55-57,共3页China Leather

基　　金：国家自然科学基金(51373158)和河南省科技创新杰出人才(144200510018)资助项目

摘　　要：研究了水提取法提取草鱼鱼鳞中的胶原蛋白,并讨论了乙二胺四乙酸脱钙对提取效果的影响。热重分析表明:脱钙4h后鱼鳞的失重情况与最终所得胶原产品的失重情况基本一致,说明脱钙4h后鱼鳞中的主要成分为胶原蛋白。所得胶原蛋白电泳条带的拖尾现象表明,存在胶原蛋白或者杂蛋白的水解。对所得产品的紫外可见光谱分析和傅立叶红外光谱分析,证实了所得产品是胶原蛋白。经过考马斯亮蓝法测得所提样品中蛋白质的含量为4.822mg/m L。The grass carp scales were decalcified before the extraction of collagen to study the influence of decalcification on the extracted collagen. The product was characterized by thermo - gravimetric analysis and SDS - PAGE. It shows that the weight loss behaviors of the sample decalcified for 4 hours is very similar to that of the resultant collagen sample. The product extracted is proved as collagen by both ultraviolet - visible spectrum and Fourier infrared spectrum. The protein content of the sample is 4. 822mg/mL, measured with Coomassie brilliant blue method.

关 键 词：鱼鳞 胶原蛋白 脱钙 表征 热重分析 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]