脉冲场凝胶电泳技术在一起食物中毒中的应用  

The application of PFGE in a food poisoning

作　　者：马鑫[1] 于烽[1] 刘艳[1] 苏静[1] 远雪梅[1] 雒婉霞[1] 

机构地区：[1]新疆自治区疾病预防控制中心,新疆乌鲁木齐830002

出　　处：《中国卫生检验杂志》2016年第4期546-548,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的利用沙门菌分子分型技术,分析不同来源的菌株之间的遗传相关性,通过同源性分析追溯新疆某县一起食物中毒发生的原因。方法对样品进行培养获得3株疑似沙门菌。通过脉冲场凝胶电泳(PFGE)技术对菌株进行鉴定及分子溯源。结果 5份患者粪便标本中有2份分离到菌株,3份食品样品中有1份分离到菌株。3株菌株初步生化鉴定结果为TSI(K/A)、产气、H2S:+;赖氨酸脱梭酶:+;血清学分型结果相同,均为O:9:12:+;H:g:m:+;H2相:-,鉴定为肠炎沙门菌。药敏检测结果显示对四环素等8种药物100%敏感,对萘啶酸100%耐药。PFGE分子分型及同源性分析,3株肠炎沙门菌的DNA图谱完全相同。结论 3株肠炎沙门菌株的DNA图谱100%同源,确认为一起由肠炎沙门菌污染了食品而引起的食物中毒。Objective To clarify of genetic correlation of strains from different isolates using molecular technologies; to define the causal agent of food poisoning in a county of Xinjiang province using homology analysis. Methods Bacteria strains were cultured for obtaining 3 Salmonella- like strains. Strains identification and molecular traceability were conducted by pulsed-field gel electrophoresis（ PFGE）. Results Two strains were isolated from five specimens of patients stool,and one strain was obtained from food samples. The preliminary biochemical identified results were as follows： TSI（ K/A）,gas,H2S： ＋,lysine decarboxylase： ＋; the results of serotyping were same： O： 9： 12： ＋; H： g： m： ＋; H2 phase：-. The identification result was Salmonella enteritidis. Susceptibility testing showed 100% sensitivity of eight kinds of tetracycline drugs,100% of nalidixic acid- resistant. With molecular typing and homology analysis of PFGE,three Salmonella enteritidis showed the same DNA fingerprinting. Conclusion The DNA fingerprinting of the three strains of Salmonella enteritidis was with 100% homology,which indicated the causal agent of food poisoning was identified as contamination caused by Salmonella enteritidis.

关 键 词：食物中毒 肠炎沙门菌 耐药性 脉冲场凝胶电泳 

分 类 号：R155.5[医药卫生—营养与食品卫生学]