溶菌酶与壳聚糖复配液抑菌作用的研究  

Antibacterial effects of muramidase and chitosan composite system

作　　者：卢小菊[1,2] 孟鸳[1] 

机构地区：[1]湖北理工学院化学与化工学院,黄石435000 [2]湖北理工学院矿区环境污染控制与修复湖北省重点实验室,黄石435000

出　　处：《中国食品添加剂》2016年第2期51-56,共6页China Food Additives

基　　金：国家自然科学基金项目(51402099);湖北省自然科学基金项目(2012FFC018);湖北理工学院校级科研项目(12xjz08R);湖北省教育厅项目(Q20083001)

摘　　要：本实验以溶菌酶和壳聚糖联合使用以增强对三种模型菌(大肠杆菌(E.coli)、金黄色葡萄球菌(St.aureus)及白色念珠菌(C.albicans))的抑菌效果。首先采用抑菌圈法考察不同浓度醋酸溶液对不同浓度壳聚糖溶液抑菌效果的影响,其次通过对倍稀释法测定壳聚糖溶液和溶菌酶溶液的最小抑菌浓度(MIC),并采用定量法确定复配液的配比,最后通过酶标法监测加入复配抑菌剂后三种模型菌的生长曲线。结果表明,1%的醋酸溶液与壳聚糖的协同抑菌效果最好;壳聚糖溶液对三种模型菌的最小抑菌浓度均为0.1562mg/m L,溶菌酶溶液对大肠杆菌、金黄色葡萄球菌和白色念珠菌的最小抑菌浓度分别为0.625mg/mL、1.25mg/mL和1.25mg/mL,而复合液对三种模型菌的抑制作用优于单独使用溶菌酶或壳聚糖;复配液对大肠杆菌和金黄色葡萄球菌的抑制作用主要表现在对数生长期,而加入材料后白色念珠菌由延迟期直接进入稳定期内。Muramidase was combined with chitosan to enhance the antibacterial effects on three model bacterias（Escherichia coli（E.coli）,Staphylococcus aureus（St.aureus）and Candida albicans（C.albicans））. First,the acetic acid concentration to dissolve chitosan was chosen by the bacteriostatic circle tested;Then,the minimal concentration of chitosan and muramidase were determined by 2-fold diluent method,and the best ratio of two component were determined by quantitative methods;finally,the growth curves of three bacteria was carried out through enzymelabeled immunosorbent assay. The results showed that chitosan with 1 % acetic acid had the best antibacterial effects. The MIC of chitosan to E.coli,St.aureus and C.albicans was 0.1562 mg/mL. The MIC of muramidase to E.coli,St.aureus and C.albicans were 0.625 mg/mL,1.25 mg/m and 1.25 mg/mL,respectively. The antibacterial effect of the compound is significantly higher than single chitosan solution and lysozyme solution. The compound inhibition effect was showed on the logarithmic phase of E.coli and St.aureu. Moreover,the growth curve of C.albicans changed directly from lag phase to stable phase.

关 键 词：溶菌酶 壳聚糖 复配液 抑菌 最小抑菌浓度 

分 类 号：TS202.3[轻工技术与工程—食品科学]