鬼针草叶蛋白最佳提取时期与方法的筛选  

Period and Method of Leaf Protein Extraction from Bidens bipinnata

作　　者：谢先中[1] 陈晓武[2] 王永树[1] 

机构地区：[1]贵州省黔西南民族职业技术学院生物工程系,贵州兴义562400 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《贵州农业科学》2016年第2期24-26,共3页Guizhou Agricultural Sciences

基　　金：黔西南民族职业技术学院科研资助项目"鬼针草野生饲草资源的综合开发与利用"(201507)

摘　　要：为了找到鬼针草叶蛋白提取最佳生长期和有效提取方法,研究了鬼针草不同生育期和不同方法对叶蛋白提取的影响。结果表明:在鬼针草4个不同生育期中采用直接加热法提取,叶蛋白提取率和沉淀物中粗蛋白含量依次降低,营养生长期(20.2 mg/g,61.36%)>现蕾期(15.9 mg/g,61.25%)>盛花期(14.7mg/g,60.97%)>结果期(12.1mg/g,54.22%),均以营养生长期为最高。在鬼针草的营养生长期,采用多种方法提取叶蛋白,叶蛋白提取率:酸化加热+聚乙二醇沉淀法(29.4mg/g)>酸化加热+乙醇沉淀法(27.9mg/g)>酸化加热+氯化钠法(24.7mg/g)>酸化加热法(23.1mg/g)>直接加热法(20.2mg/g);沉淀物中粗蛋白含量:酸化加热+乙醇沉淀法(65.35%)>酸化加热+氯化钠法(60.72%)>酸化加热+聚乙二醇沉淀法(57.13%)。The effect of different B.bipinnata growth stage and different extraction methods on leaf protein extraction rate fromB.bipinnata was studied to probe the optimum growth stage of B.bipinnata for extracting its leaf protein and effective extraction method.Results：The leaf protein rate and crude protein content in the sediment at vegetative,budding,full flowering and fruit-bearing stage are20.2mg/g and 61.36%,15.9 mg/g and 61.25,14.7 mg/g and 60.97%,and 12.1 mg/g and 54.22%respectively by using the direct heating method.When B.bipinnatais at the vegetative stage,the leaf protein extraction rate of the acidification heating ＋ polyethylene glycol,acidification heating＋ ethanol,acidification heating ＋ NaCl,acidification heating and direct heating method is 29.4 mg/g,27.9 mg/g,24.7 mg/g,23.1 mg/g and 20.2 mg/g respectively but the crude protein content in the sediment of acidification heating ＋ ethanol,acidification heating ＋ NaCl and acidification heating ＋ polyethylene glycol method is 65.35%,60.72% and 57.13%respectively.

关 键 词：鬼针草 叶蛋白 不同生育期 不同提取方法 提取率 粗蛋白 

分 类 号：S831.5[农业科学—畜牧学] Q949.95[农业科学—畜牧兽医]