不同溶剂对香芋蛋白提取及活性的影响  

Effect of Different Solvents on the Extraction and Activities of Taro Proteins

作　　者：童晶晶[1,2] 张晓元[2,3] 赖富饶[1,2] 吴晖[1,2] 余稳稳[1] 

机构地区：[1]华南理工大学轻工与食品学院,食品安全与检测研究中心,广东广州510641 [2]韶关市华工高新技术产业研究院,广东韶关512029 [3]华南理工大学工业技术研究总院,广东广州510641

出　　处：《现代食品科技》2016年第1期194-202,共9页Modern Food Science and Technology

基　　金：韶关市科技攻关项目(2013CX/N05)

摘　　要：对张溪香芋的水提物、盐提物、醇提物、Tris提取物、等电点沉淀物的蛋白质、氨基酸组成、总糖含量、总酚含量和包括抗氧化活性、凝血活性及胰蛋白酶抑制活性进行分析,以获得香芋蛋白活性较高的提取物。抗氧化性实验表明,各提取物具有一定的ABTS清除能力和羟基自由基清除能力,其中Tris提取物的ABTS清除能力(IC50=3.78 mg/m L)和羟基自由基清除能力均较高。香芋提取物均对羊血红细胞没有凝血活性,但均对兔血红细胞具有凝集活性,特异性凝集活力大小为盐提物(3.24×103 HU/mg)>Tris提取物(2.88×103 HU/mg)>等电点沉淀物(1.86×103 HU/mg)>水提物(1.41×103 HU/mg)>醇提物(0.98×103 HU/mg);五种提取物中,乙醇提取物没有胰蛋白酶抑制活性,盐提物、水提物、Tris提取物胰蛋白酶抑制活性较高,分别为134 TIU/mg、130.51 TIU/mg、113.30 TIU/mg。结果表明,Tris提取物含有较多具有高活性的蛋白质,且蛋白条带较最全。The proteinsamino acid composition, the total sugar, total phenol and the contents of water, salt, ethanol, tris（hydroxymethyl）aminomethane（Tris） and isoelectric point precipitation extracts of Zhangxi taro were determined. The antioxidant capacity, coagulating activity and trypsin inhibitory activities of the five extracts were analyzed, in order to obtain a taro protein extract with relatively high activity. The results from the experiment on antioxidant activity showed that all extracts had certain 2,2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS）·and hydroxyl radical scavenging capacities, and the Tris extract exhibited relatively strong scavenging capacities for both radicals. All taro extracts showed agglutinating activity against rabbit erythrocytes, but no coagulation activity against sheep erythrocytes. The specific hemagglutinating activities of the five extracts were as follows： salt extract（3.24 × 103 HU/mg） Tris extract（2.88 × 103HU/mg） isoelectric point precipitation（1.86 × 103 HU/mg） water extract（1.41×103 HU/mg） ethanol extract（0.98×103 HU/mg）. Among the five extracts, ethanol extract had no trypsin inhibitory activity. Salt extract, water extract, and Tris extract had higher trypsin inhibitory activities, and the corresponding values were 134 TIU/mg, 130.51 TIU/mg, and 113.30 mg/TIU, respectively. In conclusion, the Tris extract contained more proteins with high activity among the five extracts, as well as more protein bands than the others.

关 键 词：香芋蛋白 抗氧化 凝血活性 胰蛋白酶抑制剂活性 

分 类 号：TS201.21[轻工技术与工程—食品科学]