hEXO1基因单核苷酸多态性与肝细胞癌的相关性研究  

Association of hEXO1 gene polymorphism with hepatocellular carcinoma

作　　者：覃若云 谭盛葵[2] 仇小强[1] 谭超[1] 刘柳[1] 黄雄[1] 李岸花 龙静铧 廖倩[1] 杨筱筠[1] 

机构地区：[1]广西医科大学公共卫生学院流行病学教研室,广西南宁530021 [2]桂林医学院公共卫生学院流行病学教研室,广西桂林541004

出　　处：《中华疾病控制杂志》2016年第2期116-120,共5页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81160359);广西自然科学基金(2012GXNSFAA053149)

摘　　要：目的探讨肝细胞癌(hepatocellular carcinoma,HCC)遗传易感性与人类核酸外切酶1(human exonuclease 1,h EXO1)基因多态性的相关性。方法应用病例对照方法,分别选取1 199例无HCC患者作为对照组,1 196例HCC患者作为病例组。选取h EXO1基因的rs1047840、rs1776148位点进行基因分型,采取高通量Taq Man MGB实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,RT-PCR)进行基因分型。HCC发病风险与位点rs1047840、rs1776148单核苷酸多态性(single nucleotide polymorphism,SNP)的相关性应用二分类Logistic回归模型进行分析,同时研究吸烟、饮酒、乙型肝炎病毒(hepatitis B virus,HBV)感染等环境因素与位点rs1047840、rs1776148多态性的交互作用。结果位点rs1047840、rs1776148基因型在对照组、病例组的分布差异均无统计学意义(均有P>0.05)。经分层分析,rs1047840、rs1776148与HCC发病风险均无统计学关联(均有P>0.05)。交互作用分析结果显示,位点rs1047840、rs1776148基因多态性与吸烟、饮酒、HBV感染均存在交互作用。结论饮酒、吸烟、HBV感染等环境危险因素与rs1047840、rs1776148的交互作用可能与HCC发生有关。Objective To investigate the association between the human outer endonuclease 1（ human exonuclease 1,h EXO1） gene polymorphism and its susceptibility to hepatocellular carcinoma（ HCC）. Methods A case-control study was conducted in 1 199 non-tumors cases as controls,1 196 cases with HCC patients as cases. The cases and controls were frequency matched in genders,age and nations. High flux Taq Man MGB real-time quantitative polymerase chain reaction（ RT-PCR） technique was applied to genotyping for rs1047840,rs1776148 of h EXO1 gene. The binary unconditioned Logistic regression model was adopted to analyze the association between the rs1047840,rs1776148 single nucleotide polymorphisms（ SNP） and the risk of HCC,as well as to evaluate the interaction between the SNPs and the environmental factors. Results Distributions of genetypes for rs1047840,rs1776148 in the cases and the controls had no statistically significant differences（ all P 〉0. 05）. The results of stratification analyses indicated that rs1047840,rs1776148 in the cases and the controls had no statistically significant differences（ all P〉 0. 05）. Interaction analyses indicated that rs1047840,rs1776148 was significantly associated with the risk of HCC. Conclusions Interactions among polymorphisms of rs1047840,rs1776148 and drinking,smoking,HBV infection is associated with the occurrence of HCC.

关 键 词：癌 肝细胞 疾病遗传易感性 多态性 单核苷酸 

分 类 号：R730.267[医药卫生—肿瘤] R363.25[医药卫生—临床医学]