小型黄曲霉毒素检测仪的研制  

A Miniaturized Aflatoxin Analyzer Based on LED-induced Fluorescence Detector

作　　者：徐静[1] 张宁[1] 田宏哲[2] 王丹[1] 李妍[1] 刘慧颖[1] 曹际娟[1] 

机构地区：[1]辽宁出入境检验检疫局国家食品安全检测重点实验室,辽宁大连116001 [2]沈阳农业大学分析测试中心,辽宁沈阳110866

出　　处：《分析测试学报》2016年第3期299-304,共6页Journal of Instrumental Analysis

基　　金：国家质检总局资助项目(2013IK168);辽宁省农业领域青年科技创新人才资助项目(2014011)

摘　　要：针对当前黄曲霉毒素检测方法中,中低端仪器无法实现多组分定量分析,高端仪器因价格高、体积大而无法在小型实验室推广的现状,开展了黄曲霉毒素专用检测仪的研制工作。设计并搭建了基于胶束电动色谱-发光二极管诱导荧光检测系统的仪器架构,对关键光学元件等进行了组合选择,以紫外发光二极管为光源,BP 365和BP 430分别为光源和荧光滤光片,多模石英光纤传导荧光信号,光电倍增管检测信号,并对胶束电动分离系统的缓冲溶液成分等进行了优化,实现了常见6种黄曲霉毒素的基线分离。在0.1-10μg/L浓度范围内,标准溶液的浓度与荧光响应的峰面积之间呈较好的线性关系,相关系数（r）均大于0.99;峰面积的日内相对标准偏差（RSD）为2.0%-2.4%,日间RSD为5.1%-6.0%。对6种黄曲霉毒素的检出限为0.3-0.8μg/kg,加标回收率为85.3%-97.0%。该仪器具有体积小、造价低、无需衍生化反应及大量有机试剂等特点,适用于食品中多种黄曲霉毒素的同时定量检测。In the present method for aflatoxin detection,the low-end devices could not be applied in quantitation of multi-components,while the high-end devices are hard to be used in small labs due to the high cost and large volume. In this situation,a miniaturized aflatoxin analyzer was constructed based on micellar electrokinetic capillary chromatography with light emitting diode（ LED） induced fluorescence detector. The main parts of the apparatus were chosen as follows： A UV LED was used as the light source,two band pass filters including BP 365 and BP 430 were used for the excitation light and the fluorescence,respectively,ultra violet hard clad silica（ UVHCS） fiber was used to direct the fluorescence,and photomultiplier tube（ PMT） was used as the detection element. The buffer composition was investigated and the optimized conditions were found. Under the optimal conditions,baseline separation of six aflatoxins was achieved. Good linearities were obtained over the concentration range of 0. 1- 10 μg / L for six compounds with correlation coefficients larger than 0. 99. The intraRSDs were in the range of 2. 0%- 2. 4%,while the inter-RSDs were 5. 1%- 6. 0%,respectively.The limits of detection for six compounds were in the range of 0. 3- 0. 8 μg / kg,with recoveries of85. 3%- 97. 0%. With the advantages of small volume and low cost,as well as the elimination of derivatization and organic reagent,this analyzer could be widely used for the multi-aflatoxin quantitation in food analysis.

关 键 词：黄曲霉毒素 荧光 发光二极管 检测仪 

分 类 号：O652.2[理学—分析化学] TL626[理学—化学]