牛乳中嗜冷假单胞菌胞外蛋白酶的研究进展  

Progress in Research on Extracellular Proteases Produced by Psychrotrophic Pseudomonas in Milk

作　　者：徐煜[1] 游春苹[1] 

机构地区：[1]乳业生物技术国家重点实验室光明乳业股份有限公司光明乳业研究院,上海200436

出　　处：《乳业科学与技术》2016年第1期24-27,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金："十二五"国家科技支撑计划项目(2012BAD12B08);上海市科委优秀技术带头人计划项目(15XD1520300)

摘　　要：假单胞菌通常被认为是危害原料乳及其制品的主要嗜冷菌,其分泌的胞外蛋白酶具有耐热性,甚至能在超高温灭菌处理后仍有活性残留,并减短超高温灭菌乳的货架期。本文对来自牛乳中的嗜冷假单胞菌蛋白酶的研究进展做了总结,重点假单胞菌蛋白酶的催化机制研究进展,相关基因包括蛋白酶基因apr X在假单胞菌鉴别中的应用研究进展。最后讨论了嗜冷假单胞菌蛋白酶需要深入研究的方向。Pseudomonas is highlighted as predominant psychrotrophic spoilage bacteria in milk and dairy products. Its extracellular proteases are heat-resistant, and have residual activity even after ultra high temperature sterilization（UHT） treatment leading to short life of UHT milk. The current article summarizes recent studies on extracellular proteases of psychrotrophic Pseudomonas in milk, with special emphasis on their catalytic mechanisms as well as the application of protease genes including apr X to identify Pseudomonas. At last, the further research directions in this area are discussed.

关 键 词：嗜冷菌 假单胞菌 蛋白酶 基因aprX 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]