青霉素对土壤微生物群落结构的影响  

Effects of Penicillin on Soil Microbial Community Structure

作　　者：刘燕[1] 张振华[2] 鲍王波 余冉[2] 王长永[1] 

机构地区：[1]环境保护部南京环境科学研究所,江苏南京210042 [2]东南大学能源与环境学院,江苏南京210096

出　　处：《生态与农村环境学报》2016年第2期309-314,共6页Journal of Ecology and Rural Environment

基　　金：环保公益性行业科研专项(201209024);国家自然科学基金青年基金(41401363);江苏省自然科学基金青年基金(BK20130102);公益性科研院所基本科研业务费项目

摘　　要：利用Biolog-ECO技术分析不同青霉素浓度处理下土壤微生物的群落代谢活性及结构多样性,利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术分析典型微生物群落结构变化情况,探讨青霉素对土壤微生物群落的生态毒理效应。主成分分析(PCA)结果表明,高浓度青霉素(400和800 mg·kg-1)处理能快速诱导土壤中以酚酸类化合物为碳源的微生物菌群富集。DGGE结果显示,在高浓度青霉素(800 mg·kg-1)胁迫初期,土壤中微生物多样性指数(Shannon-Wiener index)显著降低(P<0.05);7 d后,主要微生物群落结构随土壤中青霉素降解又逐渐恢复。Effect of penicillin, varying in concentration, on metabolic activity and structural diversity of soil microbialcommunity was analyzed with the BiologECO technology, and changes in the soil microbial community structure were withthe DGGE （denaturing gradient gel electrophoresis） technology, so as to explore ecotoxicological effect of penicillin onsoil microbial community. PCA based on the BiologEcoplatesTM shows that penicillin high in concentration （400-800 mg·kg-1 ） may induce rapid enrichment of microbial groups that use phenolic compounds as carbon source in the soil microbialcommunity. PCRDGGE analysis reveals that in the soil treated with a high dose（800 mg·kg-1） of penicillin, ShannonWiener diversity index was significantly lowered in the first days and gradual recovered 7 days later with the penicillin inthe soil degrading.

关 键 词：青霉素 微生物多样性 BIOLOG EcoplatesTM PCR-DGGE 

分 类 号：X51[环境科学与工程—环境工程]