Manual acupuncture at the SJ5(Waiguan) acupoint shows neuroprotective effects by regulating expression of the anti-apoptotic gene Bcl-2  

作　　者：Dong Lin Li-li Lin Kyle Sutherland Chuan-hai Cao 

机构地区：[1]College of Acupuncture, Fujian University of Traditional Chinese Medicine [2]Department of Neurosurgery and Brain Repair, Morsani College of Medicine, University of South Florida

出　　处：《Neural Regeneration Research》2016年第2期305-311,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81273672,81373720;the Natural Science Foundation of Fujian Province of China,No.2014J01353;a grant from Fujian Province Health Planning Commission Project(2014)for Training Young Talents,No.2014-ZQN-JC-28

摘　　要：Acupuncture at the SJ5（Waiguan） acupoint has neuroprotective effects in cerebral infarction, but the underlying mechanism remains poorly understood. Here, we analyzed gene expression in healthy rat cerebellum using a pathway-focused DNA microarray to screen 113 genes associated with 18 signal transduction pathways. After 20 minutes of acupuncture at SJ5, the expression of Bcl-2 and Birc1 b m RNA was markedly increased. This was confirmed by real-time reverse transcription PCR. Furthermore, western blot analysis showed that Bcl-2 protein expression remained high in the cerebellum until at least 2 hours after cessation of acupuncture. These findings indicate that acupuncture at SJ5 exerts neuroprotective effects by regulating the expression of anti-apoptotic gene Bcl-2.Acupuncture at the SJ5（Waiguan） acupoint has neuroprotective effects in cerebral infarction, but the underlying mechanism remains poorly understood. Here, we analyzed gene expression in healthy rat cerebellum using a pathway-focused DNA microarray to screen 113 genes associated with 18 signal transduction pathways. After 20 minutes of acupuncture at SJ5, the expression of Bcl-2 and Birc1 b m RNA was markedly increased. This was confirmed by real-time reverse transcription PCR. Furthermore, western blot analysis showed that Bcl-2 protein expression remained high in the cerebellum until at least 2 hours after cessation of acupuncture. These findings indicate that acupuncture at SJ5 exerts neuroprotective effects by regulating the expression of anti-apoptotic gene Bcl-2.

关 键 词：nerve regeneration NEUROPROTECTION acupuncture SJ5 CEREBELLUM apoptosis signal transduction BCL-2 neural regeneration 

分 类 号：R245[医药卫生—针灸推拿学]