高效液相色谱法同时快速测定壮阳类保健品中的18种非法添加物  

Simultaneous Rapid Determination of 18 Illegal Additives in Impotence Healthy Products by HPLC

作　　者：雷毅 黄艳婷 陈林[2] 张荣[2] 

机构地区：[1]广东省食品药品检验所,广州510180 [2]广东药学院,广州510006

出　　处：《理化检验（化学分册）》2016年第3期249-254,共6页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广东省省级科技计划项目(2013B090200059);广东省中医药局课题(20132110);广州市健康产品非法添加化学成分快速检测技术重点实验室项目(2013年)

摘　　要：采用高效液相色谱法同时快速测定壮阳类保健品中的18种非法添加物。样品经乙腈(1+1)溶液超声提取后,提取液经以亚3微米填料的Agilent Poroshell 120 EC-C18色谱柱(50mm×4.6mm,2.7μm)分离,以(磷酸-三乙胺缓冲溶液)(pH 5.7)-甲醇-乙腈(70+15+15)混合液为流动相A、甲醇-乙腈(1+1)混合液为流动相B进行梯度洗脱,采用二极管阵列检测器,检测波长为230nm。18种添加物的检出限(3S/N)均为1.0 mg·kg^(-1),测定下限(10S/N)均为3.0mg·kg^(-1)。8种代表性添加物的线性范围为5.0~500 mg·L^(-1),加标回收率在88.2%~103%之间,测定值的相对标准偏差(n=6)在0.4%~1.6%之间。HPLC was applied to simultaneous rapid determination of 18 additives in impotence healthy products.The 18 additives in samples were extracted ultrasonically with acetonitrile（1＋1）solution.The extract solution were separated on an Agilent Poroshell 120EC-C18column（50mm×4.6mm,2.7μm）filled with particles of sub-3μm,with a mixture of phosphoric acid-triethylamine（pH 5.7）buffer solution,methanol and acetonitrile as mobile phase A,and a mixture of methanol and acetonitrile as mobile phase B for gradient elution.The analysts were detected with DAD detector at the wavelength of 230 nm.The detection limits and the lower limits of determination of the 18 additives were 1.0 mg·kg^（-1） and 3.0 mg·kg^（-1） respectively.The linearity ranges of 8respective additives were 5.0-500 mg·L^（-1）.Recovery rates obtained by standard addition method were in the range of 88.2%-103%,and RSDs（n=6）were in the range of 0.4%-1.6%.

关 键 词：高效液相色谱法 非法添加物 壮阳类保健品 亚3微米填料 

分 类 号：O657.7[理学—分析化学]