慢性粒细胞白血病患者Th17细胞变化的临床研究  

Clinical study on the alteration of Th17 cells in patients with chronic myeloid leukemia

作　　者：陈萍[1] 王敏[1] 李大启[1] 纪春岩[2] 马道新[2] 

机构地区：[1]山东大学附属济南市中心医院血液内科,250013 [2]山东大学齐鲁医院血液内科,济南250012

出　　处：《肿瘤研究与临床》2016年第2期82-85,共4页Cancer Research and Clinic

摘　　要：目的：探讨慢性粒细胞白血病（CML）患者外周血中Th17细胞的比例、Th17相关转录因子视黄醛酸寡核受体（RORC）的表达水平及细胞因子白细胞介素17（IL-17）浓度变化的意义。方法33例CML患者纳入研究[初诊15例，慢性期（CP）患者18例]，健康对照15名。应用流式细胞术检测Th17细胞在CML患者外周血中的比例，实时荧光定量聚合酶链反应（PCR）技术检测RORC mRNA的表达水平，酶联免疫吸附法（ELISA）检测CML患者外周血中细胞因子IL-17的浓度，分析Th17细胞与CML及临床特征的关系。结果初诊CML患者外周血Th17细胞比例[（0.71±0.41）%]低于伊马替尼治疗后疾病缓解的CML-CP患者[（4.08±0.74）%，P＜0.05]和健康对照组[（3.18±1.32）%， P＜0.05]。初诊的CML患者外周血RORC基因转录水平（0.0433±0.0405）较伊马替尼治疗后缓解的CML-CP患者（0.0861±0.0523，P＜0.05）和健康对照组（0.0910±0.0584，P＜0.05）均明显降低。初诊CML患者IL-17浓度[（1.43±0.22）pg／ml]和伊马替尼治疗后缓解的CML-CP患者外周血IL-17浓度[（1.36±0.19）pg／ml]均高于健康对照组[（1.23±0.14）pg／ml，均P＜0.05]。 Th17细胞的比例与CML患者外周血白细胞计数及bcr-abl国际标准化的比值（IS）均呈负相关。结论 Th17细胞可能在CML发病机制中起重要作用，在恶性肿瘤的免疫治疗中具有的潜在影响。Objective To investigate the role of Th17 cells in chronic myeloid leukemia （CML） pathogenesis. Methods 33 CML patients [15 newly diagnosed （ND）- and 18 chronic-phase （CP）- CML patients] and 15 healthy controls were enrolled. The percentage of Th17 cells in the peripheral blood （PB） of CML and controls were evaluated by flow cytometry. RORC mRNA expressions were examined by real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR）. The levels of IL-17 in PB of CML and controls were measured by enzyme-linked immunoadsorbent assay （ELISA）. The relationships between Th17 cells and clinical characteristics in CML were analyzed. Results The percentage of Th17 cells in PB of ND-CML patients [（0.71±0.41） %] was significantly lower than that of CP-CML patients [（4.08±0.74） %, P〈0.05] and healthy controls [（3.18±1.32） %, P〈0.05]. The mRNA level of RORC in PB of ND-CML patients （0.043 3±0.040 5） was significantly decreased compared with that in CP-CML patients （0.086 1±0.052 3, P〈0.05） and healthy controls （0.091 0±0.058 4, P〈0.05）. The levels of IL-17 in PB of ND-CML patients [（1.43±0.22） pg/ml] and CP-CML patients [（1.36±0.19） pg/ml] were slightly higher than that of healthy controls [（1.23±0.14） pg/ml, P〈 0.05]. The percentage of Th17 cells had significantly negative correlations with white blood cell counts in PB or bcr-abl （IS）. Conclusion Th17 cells may play an important role in CML pathogenesis, which has potential implication for immunotherapy of this malignancy.

关 键 词：白血病 髓样 慢性 Th17细胞 白细胞介素17 INTERLEUKIN-17 

分 类 号：R733.72[医药卫生—肿瘤]