小鼠感染大肠杆菌O127∶H6肠炎模型的建立及受激活调节正常T细胞表达和分泌活性因子的表达  

Establishment of Escherichia coli O127∶H6 Infected Mouse Model and Expression of Regulated upon Activation Normal T-cell Expressed and Secreted

作　　者：范君文[1] 隋丽华[1] 许琴[3] 刘一[1] 刘慧芳[1] 孙兆增[1] 闫亮[2] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]兰州军区乌鲁木齐总医院动物实验科,乌鲁木齐830000 [3]解放军302医院健康体检中心,北京100039

出　　处：《实验动物与比较医学》2016年第1期1-5,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的 探讨大肠杆菌O127∶H6 （Escherichia coli O127∶H6）感染BALB/c小鼠建立肠炎模型及其受激活调节正常T细胞表达和分泌活性因子（RANTES）与炎症反应的相关性.方法 通过不同剂量大肠杆菌O127∶H6灌胃的方式,建立肠炎小鼠模型（实验组）,灌胃生理盐水为对照组.取小鼠空肠标本,石蜡包埋,HE染色,观察病理变化;采用荧光定量PCR的方法,测定不同剂量组感染小鼠空肠组织的RANTES表达情况.结果 成功建立了肠炎性小鼠模型,实验组与对照组比较,空肠有明显的炎性病理变化;荧光定量PCR结果表明实验组RANTES表达量明显高于对照组.结论 通过灌胃大肠杆菌O127∶H6的方式可以建立肠炎小鼠模型,其空肠部位具有明显的病理变化;RANTES表达量的变化可以作为评价小鼠肠炎的指标之一.Objective To investigate the changes of jejunal pathology and expression of regulated upon activation normal T-cell expressed and secreted （RANTES） in BALB/c mice infected with Escherichia coli O127 ： H6. Methods Five groups of BALB/c mouse were administered intragastrically with different doses of Escherichia coli O127 ： H6 and one group administered with saline as control. The jejunum of the mouse were embedded in paraffin, and pathological changes were observed by HE staining. The jejunal RANTES expression level in the all the mice were determined by using Real-Time PCR. Results The enteritis mice model was successfully established, and significant changes of jejunal inflammatory pathology were found in model groups compared with the control group. Real-Time PCR results showed that the expression level of jejunal RANTES in the model groups were significantly higher than that of control group. Conclusion Escherichia coli O127 ; H6 can cause the changes of jejunum pathology in mice by intragastrical administration, and the RANTES expression level can be used as parameter to evaluate the in enteritis mice.

关 键 词：大肠杆菌0127:H6 病理变化 荧光定量PCR 受激活调节正常T细胞表达和分泌活性因子(RANTES) 

分 类 号：R574[医药卫生—消化系统] R-332[医药卫生—内科学]