阴外动脉灌注氨基酸对泌乳奶牛乳中主要脂肪酸组成的影响  

Effects of Intra-arterial Infusion of Amino Acids on Milk Fatty Acids Composition in Lactating Dairy Cows

作　　者：张福全[1] 敖长金[1] 哈斯额尔敦[1] 刘帅旺[1] 白晨[1] 王晛珏 高鹏[1] 张莹[1] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《畜牧兽医学报》2016年第3期529-535,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家奶业973计划(2011CB100803);教育部博士点基金(20121515120010)

摘　　要：本试验旨在研究阴外动脉灌注氨基酸对泌乳奶牛的生产性能及乳脂肪酸组成的影响。选择8头体重、胎次和泌乳阶段相近的健康中国荷斯坦奶牛,在玉米秸秆为唯一粗饲料的TMR日粮基础上,通过阴外动脉进行氨基酸灌注。试验期为27d,其中预饲期为21d,载体灌注期为3d(CSc),氨基酸灌注期为3d(CSa)。分别在两期灌注的后2d连续4次采集乳样,混合后用气相色谱法测定乳脂肪酸组成。结果表明:1)CSa组的乳脂率较CSc组提高了5.92%,两组之间差异显著(P<0.05),但两组间的乳产量及其他常规乳成分差异则不显著(P>0.05);2)阴外动脉灌注氨基酸后显著降低了乳脂中C4∶0、C6∶0、C8∶0、C10∶0、C12∶0、C14∶0和C16∶0的浓度(P<0.05),并有降低乳中C18∶1cis-9含量的趋势(P<0.10),但对乳中C18∶0、C18∶2n-6、C18∶3n-3的含量没有影响(P>0.1);3)CSa组较CSc组总脂肪酸含量下降了27.24%,显著低于CSc(P<0.05)。由此可知,在本试验条件下,泌乳奶牛阴外动脉灌注氨基酸能降低乳脂中短链脂肪酸的含量,但对长链脂肪酸的含量无显著影响。The objective of this study was to investigate the effects of intra-arterial infusion of amino acids on milk performance and milk composition of fatty acids in dairy cows.8Chinese Holstein cows,fitted with intra-arterial catheters that was terminated in right external pudendal,were used in this study.Cows were fed with TMR based on corn stover basal diet.The experiment lasted for 27 days,including 21-day preliminary period followed by 3-day carrier infusion（CSc）and3-day amino acids infusion（CSa）.Milk was collected twice daily at the last 2days of each infusion period,and mixed according to the milk production of each cow.The composition of milk fatty acids was determined by GC-FID.The results showed as follows：1）The milk fat percentage of CSa was significantly higher with 5.92%than that of CSc（P〈0.05）,whereas the remaining milk components showed no significant difference between CSa and CSc（P〉0.05）;2）The concentration of C4∶0,C6∶0,C8∶0,C10∶0,C12∶0,C14∶0and C16∶0in milk were significantly reduced after amino acids infusion（P〈0.05）,and the concentration of C18∶1cis-9in milk tended to decrease（P〈0.10）;3）Compared with CSc,AA infusion significantly decreased the total fattyacids concentration by 27.24%in milk（P〈0.05）in CSa group.These results indicate that intraarterial infusion of amino acids can decrease the content of short-and medium-chain fatty acids in milk,which did not affect long-chain fatty acids content in milk.

关 键 词：泌乳奶牛 阴外动脉 氨基酸 灌注 乳脂肪酸组成 

分 类 号：S823.91[农业科学—畜牧学] S815.4[农业科学—畜牧兽医]