响应面法优化红花酢浆草抑菌活性物质的提取工艺研究  

Extraction process optimization of antibacterial active substance from Oxalis corymbosa by response surface method

作　　者：张玉枫[1] 张金华[1] 黄瑶[1] 李开秀[1] 杨莉娜[2] 王丹[1] 

机构地区：[1]成都医学院生物医学系,四川成都610500 [2]中国科学院环境与应用微生物重点实验室,四川成都610041

出　　处：《中国测试》2016年第3期53-58,共6页China Measurement & Test

基　　金：四川省教育厅重点项目(16ZA087);国家级大学生创新训练项目(201513705006);中国科学院环境与应用微生物重点实验室开放基金项目(KLCAS-2014-04)

摘　　要：为建立红花酢浆草抑菌活性物质的最佳提取工艺,在单因素实验的基础上,选取提取时间、乙醇体积分数和固液比为自变量,抑菌圈直径为响应值,建立数学模型,求解得到最佳提取条件和理论最大抑菌圈并验证。结果显示,红花酢浆草抑菌活性物质的最佳提取工艺为:乙醇体积分数73%,提取时间78 min,固液比1∶31 g/m L。此时,红花酢浆草醇提取物拮抗金黄色葡萄球菌的抑菌圈直径为30.17 mm,较优化前提高67.61%。响应面优化法不仅效率高,而且可靠性强,可准确预测抑菌圈直径。同时,证明红花酢浆草的醇提取物具有较为广泛的抑菌谱。An optimum process is designed for extracting antibacterial active substance from Oxalis corymbosa. The extraction time, ethanol concentration and solid-liquid ratio are selected as independent variables based on single factor experiment and the diameter of the inhibition zone is considered as response value. After that, a mathematical model is established to calculate and verify the optimal extraction conditions and the theoretically maximum inhibition zone. The optimum extraction process is shown as follows：73% ethanol concentration,78 min extraction time,1∶31 g/m L solid-liquid ratio. Under these conditions,the maximum antibacterial diameter is 30.17 mm,which has been increased by 67.61% after optimization. The response surface optimization method introduced in this paper is not only efficient but also reliable; with it, the inhibition zone diameter has been accurately predicted. Besides,the alcohol extract of Oxalis corymbosa has been proved to have a wide range of antibacterial spectra.

关 键 词：红花酢浆草 抑菌活性 响应面法 抑菌谱 提取工艺 

分 类 号：TS202.3[轻工技术与工程—食品科学]