羊乳中类胰岛素生长因子Ⅰ的特性  

Investigation on the characteristics of IGF-Ⅰ in goat milk

作　　者：侯院林[1] 张富新[1] 李龙柱 于玲玲[1] 云丹[1] 雷飞燕[1] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]西安市碑林区食品药品监督管理局,陕西西安710000

出　　处：《食品与发酵工业》2016年第3期30-35,共6页Food and Fermentation Industries

基　　金："十二五"农村领域国家科技计划项目(2013BAD18B00);农业部公益性行业(农业)科研专项(201103038);陕西省农业攻关项目(2014K01-17-05)

摘　　要：采用双抗体夹心酶联免疫法研究了温度、p H、紫外线以及金属离子等因素对羊乳中类胰岛素生长因子Ⅰ(insulin-like grouth factor-Ⅰ,IGF-Ⅰ)浓度的影响。研究表明:羊乳中IGF-Ⅰ在20℃时浓度最高,达到(29.91±0.91)ng/m L(P<0.05),在40℃时具有较高的热稳定性;IGF-Ⅰ在p H=4时浓度最高,达到(28.53±1.11)ng/m L(P<0.05),在p H值为6~8时稳定性较高;紫外线照射会造成IGF-Ⅰ的浓度显著降低(P<0.05)。Na^+、Ca^(2+)、Al^(3+)、Zn^(2+)等金属离子对IGF-Ⅰ的浓度具有抑制作用,而Mg^(2+)、Fe^(2+)对IGF-Ⅰ无明显影响,低浓度的Fe^(3+)、Cu^(2+)对IGF-Ⅰ的浓度具有抑制作用,而高浓度的Fe^(3+)、Cu^(2+)对IGF-Ⅰ具有激活作用。通过研究,评估温度、p H、紫外线以及金属离子对羊乳中IGF-Ⅰ浓度的影响,为生产具有更高IGF-Ⅰ浓度的功能活性羊乳产品提供依据和指导。Effects of Insulin-like growth factor-Ⅰ characteristic factors in goat milk were studied to lay the theoretical foundation for the further development of active functional goat products which contained higher IGF-Ⅰ concentrations. Effects of temperature,p H,ultraviolet and metal ion on IGF-Ⅰ concentrations in goat milk were studied by double antibody sandwich enzyme-linked immunosorbent assay. The results showed that IGF-Ⅰ concentrations reached the highest level to（ 29. 91 ± 0. 91） ng/m L at 20 ℃ in goat milk（ P〈0. 05） and had higher thermal stability at 40℃. IGF-Ⅰ concentrations were the highest at p H4 with 28. 53 ± 1. 11 ng/m L（ P〈0. 05）,and more stable at p H6 ~ 8.IGF-Ⅰ concentrations in goat milk decreased severely on the condition of ultraviolet radiation（ P〈0. 05）. IGF-Ⅰ concentrations would be inhibited if added Na^＋,Ca^2＋,Al^3＋,Zn^2＋in goat milk while Mg^2＋,Fe^2＋had no effect on this.IGF-Ⅰ concentrations were inhibited at lower concentrations of Fe^3＋and Cu^2＋. However,it could be activated at higher concentrations of Fe^3＋and Cu^2＋. The research evaluate the effect of temperature,p H ultraviolet and metal ion on IGF-Ⅰ concentrations in goat milk. It provides the basis and guidance for the active functional goat products which contained higher IGF-Ⅰ concentrations.

关 键 词：羊乳 类胰岛素生长因子Ⅰ 特性 双抗体夹心酶联免疫法 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]