植物转基因技术在高校本科生实验课程中的应用及方案设计  

The Application and Experimental Project Design of Plant Transgenic Technology in the Undergraduate Experimental Course

作　　者：宋宏涛[1] 李万杰[2] 向本琼[1] 聂频文[1] 

机构地区：[1]北京师范大学生命科学学院实验教学中心,北京100875 [2]北京师范大学细胞增殖及调控生物学教育部重点实验室,北京100875

出　　处：《中国细胞生物学学报》2016年第2期193-201,共9页Chinese Journal of Cell Biology

基　　金：北京师范大学教学建设与改革项目(批准号:12-02-16)资助的课题~~

摘　　要：植物转基因技术是生物学研究的常用技术,也是高校生物学系本科生实验教学的重要内容之一。该文结合不同的植物转基因技术手段,对原有的植物转基因技术在实验课程中的应用方案进行了重新整合设计,从转基因植株的获得、筛选到多层次鉴定转基因效果等,科学完整地设计了综合实验课程,探究了实验的实施和可行性,并给出了具体的课程实施建议。该教学实验设计也为高校生物学实验课程改革作了全新的探究和尝试。The plant transgenic technology is commonly used in biology research, and it is one of important contents in undergraduate experimental teaching courses. In this paper, the experimental project for plant transgenic technology is re-integrated and designed by combing multiple plant transgenic technologies. The comprehensive experimental project includes the production of transgenic plants, screening of transgenic plants and multi-level identification of transgenic effects. We also explore the feasibility of implementation for the experimental project, and give some detailed suggestions for its implementation. The experimental teaching design can be considered as a new exploration and attempt for the reform of the undergraduate biology experimental course.

关 键 词：植物转基因 综合实验 实验课程改革 

分 类 号：Q943.2-4[生物学—植物学] G652[文化科学—教育学]