Stathmin对卵巢癌C13K细胞侵袭迁移能力的影响  

The effect of Stathmin on invasion and migration of C13K cells in ovarian cancer

作　　者：张军港[1] 石英[2] 

机构地区：[1]浙江省人民医院肝胆胰外科,浙江杭州310014 [2]浙江省人民医院妇产科,浙江杭州310014

出　　处：《中国卫生检验杂志》2016年第6期778-781,共4页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金青年基金(81502482);浙江省自然科学基金(LQ14H160017);浙江省医药卫生科研项目(2015KYB026)

摘　　要：目的探讨微管调节蛋白Stathmin对卵巢癌C13K细胞侵袭和迁移性的影响及机制研究。方法选择C13K细胞为实验对象,应用siRNA靶向沉默Stathmin,利用实时定量PCR(qRT-PCR)方法检测转染48 h后转染效果,transwell法和细胞划痕实验测定细胞侵袭和迁移能力变化,qRT-PCR检测侵袭相关基因MMP2和MMP9的变化。结果与空白对照组和阴性对照组相比较,Stathmin siRNA明显降低了C13K细胞中Stathmin mRNA的表达量,StathminsiRNA组侵袭的细胞数和细胞损伤愈合的速度明显降低,Stathmin-siRNA组MMP2和MMP9表达量明显降低。结论沉默Stathmin表达能有效抑制卵巢癌细胞的侵袭迁移能力,其机制可能与抑制MMP2和MMP9的表达有关,为卵巢癌的治疗前景增加新的希望。Objective To explore the roles of Stathmin gene on the invasion and migration of C13 K cells in human ovarian cancer,as well as to analyze the mechanisms. Methods C13 K cells were collected as the subjects,with the application of siRNA targeted Stathmin,the interference result was verified by qRT- PCR in 24 h after transfection. The cell invasion and migration were determined by transwell and wound healing assay. The change of invasion- related genes MMP2 and MMP9 was measured by qRT- PCR. Results Compared with blank control group and negative control group,the Stathmin mRNA relative content in Stathmin- siRNA group significantly reduced,and the number of invasive cells and the healing speed of C13 K cell injury significantly reduced. After the Stathmin siRNA,the relative expression of MMP2 and MMP9 in Stathmin- siRNA group was significantly lower. Conclusion Silencing Stathmin expression can effectively inhibit the invasion and migration of ovarian cancer cells,and its mechanism may be related to inhibiting the expression of MMP2 and MMP9,which brings the new hope for the treatment of ovarian cancer.

关 键 词：卵巢癌 STATHMIN SIRNA 侵袭 迁移 

分 类 号：R737.31[医药卫生—肿瘤]