Kaposi肉瘤相关疱疹病毒感染诱导LYN表达在其发病中的作用!  

Kaposi's Sarcoma Associated Herpesvirus Induces LYN Overexpression in Kaposis Sarcoma

作　　者：张芳[1] 陈颖[2] 郭峰[2] 徐益明[3] 谭晓华[4] 杨磊[4] 李锋[1] 姬玉[1] 李冬妹[1] 

机构地区：[1]石河子大学医学院,新疆石河子832002 [2]People's Hospital of Xinjiang Uygur Autonomous Region [3]Hospital of Bayingolin Mongol Autonomous Prefecture [4]Medical College of Hangzhou Normal University

出　　处：《中国皮肤性病学杂志》2016年第4期341-344,共4页The Chinese Journal of Dermatovenereology

基　　金：新疆生产建设兵团应用基础研究(2015AG015);高层次人才科研启动资金专项(RCZX201332)

摘　　要：目的探讨Kaposi肉瘤相关疱疹病毒(kaposi’s sarcoma associated herpesvirus,KSHV)感染对v-yes-1Yamaguchi肉瘤病毒相关同源癌基因(v-yes-1 Yamaguchi sarcoma viral related oncogene homolog,LYN)表达的影响,及其在卡波氏肉瘤(kaposi’s sarcoma,KS)发病机制中的作用。方法采用酶灌注法原代培养脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs),运用免疫细胞化学检测CD34和CD31以验证HUVECs。从BCBL-1中提取KSHV病毒感染HUVECs,显微镜下观察细胞形态并拍照,PCR扩增KS330_(233)证实感染成功。采用Western blot检测LYN表达及p-PI3K和p-AKT水平。结果HUVECs中CD 31胞膜阳性和CD 34胞浆阳性,说明酶灌注法取得的细胞为HUVECs。KSHV感染HUVECs后细胞变为长梭形,LYN表达增高,同时p-PI3K和p-AKT水平也增高。结论 KSHV感染HUVECs促进LYN表达,且可能通过促进p-PI3K和p-AKT水平增高而活化PI3K/AKT信号通路。Objective To analyze the function of Kaposi＇s Sarcoma associated herpesvirus （KSHV） infection in the expression of v-yes-1 Yamaguchi sarcoma viral related oncogene homolog （lyn） and reveal the mechanism in Kaposis Sarcoma （KS）. Methods Primary human umbilical vein endothelial cells （HUVECs） were cuhivated by enzyme infusion method and immunocytochemistry （ICC） was used to detect CD31 and CD34 to verify HUVECs. KSHV were extracted from BCBL-1 cells, then used to infect HUVECs. We observed the cell morphology variation using microscope and recorded imagines. PCR was used to amplificate KS330233 fragment to verify the infection. Western blot was used to detect the level of LYN, p-PI3K and p-AKT. Results HUVECs were cytomembrane staining for CD31 and cytoplasmic staining for CD34, which verified that the primary cultivated cells were HUVECs. After KSHV infected HUVECs, the cells showed long spindle morphology. The expression of LYN was increase and the expression of p-PI3K and p-AKT was also increased. Conclusion KSHV promoted overexpression of LYN in KSHV de novo infected HUVECs and increased the level of p-PI3K as well as p-AKT in PI3K/AKT signaling pathway.

关 键 词：Kaposi肉瘤相关疱疹病毒 LYN KAPOSI肉瘤 P13K/AKT 

分 类 号：R739.5[医药卫生—肿瘤]