液相^(31)P-NMR快速检测鱼子酱中磷脂  

Determination of Phospholipids in Caviar by Solution ^(31)P-NMR

作　　者：张明霞[1] 庞建光[2] 呼秀智[3] 姬红萍 

机构地区：[1]河北工程大学理学院,河北邯郸056038 [2]河北工程大学建筑学院,河北邯郸056038 [3]河北工程大学成人教育学院,河北邯郸056021 [4]河北邯郸县农牧局,河北邯郸056006

出　　处：《食品科学》2016年第6期153-156,共4页Food Science

基　　金：河北省科技支撑计划项目(14227201D);河北省邯郸市科技研究与发展计划项目(1122104039-2)

摘　　要：建立液相^(31)P-核磁共振光谱法快速检测鱼子酱食品中磷脂的分析方法。样品匀浆后氯仿和甲醇混合溶剂提取其中的全脂,无需分离直接溶于氘代试剂后进行核磁分析。该方法回收率为96%~102%,相对标准偏差不超过5%。采用本方法对4种品牌鱼子酱食品检测均发现6种磷脂组分存在,且圆鳍鱼籽中磷脂酰胆碱和溶血磷脂酰胆碱总量均占总磷脂量的80%以上,而鲟鱼籽中磷脂酰肌醇含量远高于圆鳍鱼籽。其他3种磷脂磷脂酰乙醇胺、鞘磷脂和甘油磷脂酸在2种取材的鱼子酱中含量相差不多。An improved method for the determination of phospholipids in caviar by solution ^31P-nuclear magnetic resonance（^31P-NMR） was studied. The lipids in the sample were extracted with chloroform and methanol, dissolved in deuterated reagent and then analyzed by ^31P-NMR without separation. The average recoveries of the established method were 96%–102%, with relative standard deviation（RSD） not exceeding 5%. Phospholipids of 4 commercial brands of caviar were analyzed and the relative distribution of phospholipid components was measured in mole percentage. It was found that phosphatidylcholine and lysophosphatidylcholine accounted for more than 80% of the total phospholipids in lumpfish roe, and phosphatidylinositol content in sturgeon roe was much higher than that of lumpfish roe. The contents of phosphatidylethanolamine, sphingomyelin and phosphatidic acid were similar in both fish roes.

关 键 词：^31P-核磁共振光谱法 磷脂 鱼子酱 检测 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]