响应面优化复合酶提取夏枯草多糖  

Response Surface Optimization of Complex Enzyme Extraction Polysaccharides from Prunella Vulgaris

作　　者：金辰光 陈瑞战[1] 谭莉[1] 常清泉[1] 陆娟[1] 章雯雯[1] 

机构地区：[1]长春师范大学化学学院,吉林长春130032

出　　处：《长春师范大学学报》2016年第2期49-53,共5页Journal of Changchun Normal University

基　　金：吉林省教育厅"十二五"科学技术重点项目([2014]258;[2015]355);长春师范大学研究生教育创新计划(cscxy2014003)

摘　　要：本文探索和优化一种高效的复合酶解技术从夏枯草中提取多糖。在单因素试验的基础上,选择酶添加量(A)、提取温度(B)和溶液p H值(C)三因素三水平的Box-Behnken Design(BBD)试验设计,响应面优化提取工艺。得到最佳提取条件:纤维素酶浓度0.4%、果胶酶浓度0.8%、木瓜蛋白酶浓度0.8%,提取温度61℃,p H 6.2。在此条件下夏枯草粗多糖的提取得率为11.57±0.48%(n=3),与预测得率(12.01%)基本一致。说明优化得到的工艺合理、可行,复合酶提取得率高、能耗低,是一种很有希望的提取方法。In this study,an efficient complex enzyme extraction（ CEE） technology was developed and optimized to extract polysaccharides from Prunella vulgaris. On the basis of single factor test,response surface methodology（ RSM） based on a three- factor three-level Box- Behnken Design（ BBD） was employed to optimize the extraction conditions including enzyme concentration,extraction temperature and p H. The optimal extraction conditions were as follows： cellulase 0. 4%,pectinase 0. 8%,papain 0. 8%,extraction temperature 61℃,p H 6. 2. Under these conditions,the experimental yield was 11. 57 ± 0. 48%,which is well in close agreement with the value predicted（ 12. 01%） by the model. These results demonstrated that the optimal extraction technology is reasonable and feasible.The CEE is a promising extraction method that offers higher yield and lower energy cost.

关 键 词：夏枯草 多糖 复合酶 优化 

分 类 号：O629.12[理学—有机化学]