黔产茶叶中儿茶素类成分的HPLC分析研究  

Study of Content Determination of Catechins in Guizhou-tea

作　　者：左爱萍 张明[2] 王道平[2] 陶晨 杨小生 

机构地区：[1]贵州威门药业股份有限公司,贵州贵阳550018 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [3]黔南民族医学专科学校,贵州都匀558000

出　　处：《信阳师范学院学报（自然科学版）》2016年第2期209-211,共3页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：贵州省优秀科技教育人才省长资金项目(黔省专合字(2011)12号)

摘　　要：建立一种同时测定黔产茶叶中四种儿茶素(表没食子儿茶素没食子酸酯、表儿茶素、没食子儿茶素没食子酸酯和表没食子儿茶素)含量的高效液相色谱分析方法.采用Agilent 1100高效液相色谱仪、Welch色谱柱ultimate XB-C18(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸为流动相进行梯度洗脱,在流速1 m Lmin-1、柱温35℃、检测波长278 nm的条件下测定了该四种儿茶素的含量,回收率均在98.4%~102.00%之间,各组分回归方程的相关系数均在0.99996以上.该方法选择性好,灵敏度高,重复性好,检测快速准确,适合用于茶叶中儿茶素含量的测定.To establish a HPLC method for simultaneously determination the content of four kinds of catechins（ EGCG,EC,ECG,EGC） from leaves in Guizhou-tea.Chromatographic separation was performed with ultimate XB-C18column（ 4.6 mm×250 mm,5 μm） by using the mobile phase gradient elution of methanol-0.1% phosphoric acid mixture,the flow rate is 1 m L·min^（-1）,the column temperature is 35℃ and the detection wavelength is 278 nm.Under the optimum condition,four catechins（ EGCG,EC,ECG and EGC） were separated and determined.This developed method was successfully used to measure and analyze four catechins in Guizhou-Tea.The averagerecoveries were 98.4% ~ 102.00%,all the correlation coefficients of regression equation of each component is above 0.99996.

关 键 词：茶叶 儿茶素 高效液相色谱法 

分 类 号：S571.1[农业科学—茶叶生产加工]