产氢菌的分离鉴定及发酵性能  

Screening,identification of hydrogenogen and the research of its fermentation performance

作　　者：张存胜 王文娟[1] 王振斌[1] 邵淑萍[1,3] 马海乐[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]固体废物处理与环境安全教育部重实验室(清华大学),北京100084 [3]青岛啤酒股份有限公司,山东青岛266100

出　　处：《化工进展》2016年第4期1184-1189,共6页Chemical Industry and Engineering Progress

基　　金：中国博士后科学基金(2014M561589);江苏省自然科学基金青年基金项目(BK20150487);中联环SWMES教育部重点实验室开放基金(SWMES 2015-11);江苏高校优势学科建设工程项目

摘　　要：为了获得高性能的产氢菌,从碱处理后活性污泥中分离纯化得到两株产氢菌(H-1和H-2),生物学鉴定表明两株菌均为Enterobacter种属,H-1菌为Enterobacter cancerogeous HG6 2A种属,而H-2与Enterobacter homaechei 83的关系最亲近。发酵实验结果表明,将H-1和H-2菌液进行1∶1混合时发酵性能最佳,对应的发酵时间和产氢量分别为33h和861m L/L,混合发酵克服了H-1菌单独发酵氢产量低和H-2菌发酵时间长的缺陷。发酵液中主要挥发性脂肪酸(VFA)为乙酸和丁酸,表明两株菌的发酵类型均为丁酸型发酵。由于两株菌的协同作用,混合发酵初期VFA的累积速率降低,提高了发酵体系的稳定性。In order to obtain high performance hydrogen-producing bacteria,two strains（H-1 and H-2） were screened from activated sludge which was pretreated by alkali. Biological identification showed that the two strains were of Enterobacter species. H-1 was Enterobacter cancerogeous HG6 2A species and H-2 had the closest relationship with Enterobacter homaechei 83. Results showed that the best performance could be achieved from co-fermentation at the ratio of 1 ∶ 1（H-1 ∶ H-2）. The corresponding fermentation time and hydrogen yield were 33 h and 861 m L/L,respectively. The defects of lower hydrogen yield by H-1 and longer fermentation time by H-2 were overcome by co-fermentation. The main volatile fatty acids（VFA） in the broth were acetate and butyrate,indicating that the fermentation of the two strains were both butyrate type. Due to the synergistic effect of the two strains,the accumulation rate of VFA reduced at the initial of fermentation. As a result,the stability of the system was enhanced.

关 键 词：产氢菌 16S RDNA 制氢 发酵 协同作用 

分 类 号：X712[环境科学与工程—环境工程] TK91[动力工程及工程热物理]