一株凡纳滨对虾源鳗弧菌群体感应信号分子的检测  

Detection of Quorum Sensing Signal Molecules in Vibrio anguillarum Isolated From Litopenaeus vannamei

作　　者：潘玉荣[1] 张彩丽[1] 朱素芹[1] 孙秀娇 曾名湧[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《生物技术通报》2016年第3期142-147,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31071613)

摘　　要：旨在检测从凡纳滨对虾体内选取的一株鳗弧菌的群体感应信号分子。利用报告菌株结合薄层层析法鉴定鳗弧菌(Vibrio anguillarum,VA)分泌的高丝氨酸内酯类(AHLs)信号分子,利用哈维氏弧菌V.harveyi JMH597和V.harveyi JAF375作为报告菌株分别检测了VA分泌的AI-2和CAI-1信号分子活性与细菌密度的关系。结果表明,VA能分泌3种类型的信号分子:AHLs信号分子、AI-2信号分子和CAI-1信号分子,其中分泌的AHLs有N-3-羟基-己酰基-高丝氨酸内酯(3-OH-C6-HSL)、N-3-辛酰基-高丝氨酸内酯(C_8-HSL)和N-3-氧代-辛酰基-高丝氨酸内酯(3-oxo-C_8-HSL)。VA分泌的AHLs、AI-2和CAI-1均具有密度依赖性。The aim of the present study was to detect and analyze quorum sensing of Vibrio anguillarum（VA）isolated from spoiled Litopenaeus vannamei. AHLs activities of VA were detected by reporter strains（Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136）combined with TLC method. Report strains V. harveyi JMH597 and V. harveyi JAF375 were used to detect the activity of AI-2 and CAI-1. The result indicated that AHLs（3-OH-C6-HSL, C_8-HSL and 3-oxo-C8-HSL）、AI-2 and CAI-1 were secreted by VA andthe activity of the signal molecules were density-dependent.

关 键 词：鳗弧菌 群体感应 AHLs信号分子 AI-2类信号分子 CAI-1类信号分子 

分 类 号：S941.4[农业科学—水产养殖]