上调基因-11(URG11)生物信息学与功能分析  

Function Analysis of Up-regulated Gene(URG11) Based on Its Bioinformatics

作　　者：姚杨[1] 苏杰[1] 刘凯歌[1] 徐锐[1] 

机构地区：[1]西安医学院第一附属医院,西安710077

出　　处：《生物技术通报》2016年第3期203-208,共6页Biotechnology Bulletin

基　　金：西安市科技计划项目(SF1418(6));西安医学院科研项目(12FZ15);西安医学院附属医院科研项目(XYFY11-14)

摘　　要：应用基因芯片技术获取以稳定转染HBx基因的肝癌细胞Hep G(Hep G22-X)Y,以及非转染的肝癌细胞Hep G 2的差异表达基因,利用生物信息学方法对其进行初步分析表明,该蛋白基因编码673个氨基酸,预测分子量为17.06 k D,理论等电点为4.83,定位于细胞核,具有转录调控、生长因子、信号传导的功能,同源性分析结果表明,其碱基序列与已经报道的其他12个物种的相似率为76%-97%,且符合种属之间的进化关系。This work was to obtain differentially-expressed genes with hepatoma cell Hep G（Hep G2-X）Y of stable transfected HBx and non-transfected hepatoma cell Hep G 2 using gene chip technology. The preliminary bioinformatic analysis demonstrated that the gene of the protein encoded 673 amino acids with a predicted molecular weight of 17.06 k D and p I 4.83. URG11 were mainly localized in the nuclear and played the role in transcription regulation,growth factor and signal transducer,and it showed 76%-97% identity with the others reported 12 species,as well as was consistent with the evolutionary relationship between species.

关 键 词：乙肝相关性肝癌 生物信息学分析 URG11 HBX 

分 类 号：R735.7[医药卫生—肿瘤] Q811.4[医药卫生—临床医学]