李斯特菌噬菌体裂解酶的研究进展  

Research Progress in Listeria Phage Endolysins

作　　者：刘珊娜[1] 范志华[1] 孙溪[1] 付荣霞[1] 吴海清[1] 

机构地区：[1]天津农学院食品科学与生物工程学院,天津市农副产品深加工技术工程中心,天津300384

出　　处：《天津农学院学报》2016年第1期62-65,共4页Journal of Tianjin Agricultural University

基　　金：天津市科技支撑计划项目“基于噬菌体蛋白的李斯特菌快速检测技术及试纸的研发”(14ZCZDNC00003)

摘　　要：单核细胞增生李斯特菌是重要的食源性致病菌,可通过污染的食品进入人体,引发食品安全事件。噬菌体作为李斯特菌生物防治的有效手段,能够特异性识别菌体细胞,造成细菌裂解死亡。噬菌体发挥抑菌活性的关键在于其双链DNA所编码的裂解酶(肽聚糖水解酶类)能够在增殖过程的后期有效裂解宿主菌的细胞壁,将子代噬菌体释放到胞外环境中。本文综述了李斯特菌噬菌体裂解酶的种类、作用原理和结构特征,并探讨了裂解酶的应用领域和未来发展方向。Listeria monocytogenes is an important genus of food-borne pathogens. It may transmit into human body through contaminated food and cause food safety issue. Bacteriophages are effective means for Listeria bio-control, which can specifically recognize bacterial cells and lead to bacterial lysis. The key to antibacterial activities of bacteriophages relies on double stranded DNA-encoded endolysins, the murein enzymes which degrade host cell wall and result in release of phage progeny to the extracellular environment at the end of the phage lytic multiplication cycle. This article reviews the category of Listeria phage endolysins, the mechanism and structural feature of them, and discusses the application area and direction for development of endolysins in the future.

关 键 词：李斯特菌 噬菌体 裂解酶 研究进展 

分 类 号：S852.61[农业科学—基础兽医学]