大亚湾浮游细菌的变性梯度凝胶电泳分析  

DNA fingerprints of bacterioplankton in Daya Bay using PCR-DGGE

作　　者：王朝晖[1] 乔倩[1] 江晓亮[1] 郭鑫[1] 

机构地区：[1]暨南大学生态学系水体富营养化与赤潮防治广东普通高校重点实验室(暨南大学),广州510632

出　　处：《环境科学学报》2016年第4期1212-1218,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目(No.41276154;41476132)~~

摘　　要：于2010年12月以及2011年6月采集了大亚湾9个站点的表层水样,利用PCR-变性梯度凝胶电泳（PCR-DGGE）技术对浮游细菌DNA指纹进行了分析.结果显示,大亚湾海域浮游细菌DNA指纹比较丰富,冬季和夏季样品中分别共得到32条和33条条带,每个样品得到的条带数为18-25条.DNA指纹的Shannon-Weaver多样性指数（H＇）和Pielou均匀度（J）的变化范围分别为2.48-2.88和0.80-0.93.DNA指纹条带数与温度和盐度明显正相关,而与p H、DO、透明度呈明显负相关关系.结果说明PCR-DGGE技术可以在一定程度上反映细菌的群落结构和多样性,但需要与其他技术相结合方能较全面反映细菌的结构与功能.DNA fingerprints of bacterioplankton in Surface water of Daya Bay,the South China Sea,were studied using PCR-denaturing gradient gel electrophoresis（ PCR-DGGE）. Surface water samples were collected twice in December 2010（ winter） and June 2011（ summer）. A total of 32 and 33 DGGE bands were observed in the winter and the summer samples,respectively,and 18 - 25 of which were in both samples,indicating bacterioplankton community of Daya Bay were in high diversity. The Shannon-Weaver diversity index（ H＇） and Pielow evenness（ J） were 2. 48 - 2. 88 and 0. 80 - 0. 93,respectively. DNA fingerprints exhibit significant positive correlations with water temperature and salinity,while negatively correlated with p H,DO and transparency. The results suggest that PCR-DGGE might reflect the bacterioplankton community structure and diversity. However the combination of other techniques are required to completely understand the structure and functions of bacterioplankton.

关 键 词：浮游细菌 PCR-DGGE DNA指纹图谱 大亚湾 

分 类 号：X172[环境科学与工程—环境科学]