黄腐酸对雨生红球藻(Haematococcus pluvialis LUGU)虾青素积累的影响  

Effects of fulvic acid on astaxanthin accumulation of Haematococcus pluvialis LUGU

作　　者：尚敏敏 赵永腾 李涛[1] 赵鹏[1] 徐军伟[1] 余旭亚[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《食品工业科技》2016年第8期225-228,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(21266013)

摘　　要：通过形态学以及18S r RNA的分析,筛选鉴定出了一株新的雨生红球藻Haematococcus pluvialis LUGU,并以其作为研究对象,研究了不同质量浓度的黄腐酸(FA)对胁迫培养条件下细胞生长和虾青素产量的影响。结果表明,5 mg/L和10 mg/L的FA不仅促进了藻细胞的生长(生物量浓度分别比对照组增加了4.26%和10.58%),而且能够显著提高虾青素的含量(最大含量分别达到20.82和12.23 mg/L)。其中,5 mg/L的FA诱导效果最为明显,虾青素产量(37.46 mg/g)比对照组(20.90 mg/g)提高79.23%。实验表明,黄腐酸在适当浓度下能够显著促进藻细胞内虾青素的积累,可作为一种有效的诱导子应用于虾青素的生产。A novel strain identified by the morphology and 18 S r RNA namely,Haematococcus pluvialis LUGU,was used to explore the effect of fulvic acid（FA） on the growth of algae and the production of astaxanthin in this study. As a result,the biomass of H. pluvialis increased by 4.26% and 10.58% than controls and the astaxanthin content reached 20.82 and 12.23 mg/L in 5 and 10 mg/L FA-treated culture,respectively. Particularly,5 mg/L FA enhanced 79.23% with 37.46 mg/g in the production of astaxanthin more than that of control group（20.90 mg/g）. The result indicated that FA was a effective factor to enhance the production of astaxanthin at appropriate concentration.

关 键 词：雨生红球藻 虾青素 黄腐酸(FA) 

分 类 号：Q949.212[生物学—植物学]