气相色谱-串联质谱法快速检测玉米粉中乙草胺、异丙甲草胺和三唑酮残留  

Simultaneous determination of acetochlor, metolachlor and triadimefon residue in corn flour by gas chromatography-tandem mass spectrometry

作　　者：叶倩[1,2] 路大海 邓义才[1,3] 黄健祥[1,3] 骆冲[1,2] 

机构地区：[1]广东省农业科学院农产品公共监测中心,广州510640 [2]农业部农产品质量安全检测与评价重点实验室,广州510640 [3]农业部农产品质量安全风险评估实验室(广州),广州510640

出　　处：《农药学学报》2016年第2期268-272,共5页Chinese Journal of Pesticide Science

基　　金：国家农产品质量安全风险评估项目(GJFP201500102);公益性行业(农业)科研专项(201303088)

摘　　要：建立了玉米粉中乙草胺、异丙甲草胺和三唑酮残留检测的气相色谱-串联质谱（GCMS/MS）分析方法。样品采用乙腈浸提3 h、过两次0.22μm尼龙66微孔过滤膜后,以气相色谱-串联质谱EI离子源,多反应监测模式（MRM）检测。结果表明：在0.005～1 mg/L范围内,3种供试农药的质量浓度与其相应的峰面积间呈良好的线性关系,决定系数（R2）均大于0.999。在0.025、0.050和0.25 mg/kg 3个添加水平下,3种农药的平均回收率为95%～101%,相对标准偏差（RSD）为0.7%～2.2%（n=6）。3种农药在玉米粉中的定量限（LOQ）分别为0.05、0.05和0.2μg/kg。该方法准确、灵敏、简单,适用于同时快速检测玉米粉中乙草胺、异丙甲草胺和三唑酮的残留量。A gas chromatography-tandem mass spectrometry（GC-MS/MS） method was established for the simultaneous determination of acetochlor, metolachlor and triadimefon residue in corn flour. The samples were extracted with acetonitrile for 3 h, and the upper acetonitrile layer was filtered with0.22 μm nylon membrane.The samples were then determined by GC-MS/MS. The results showed that the calibration curve was linear in the range of 0.005-1.0 mg/L. The determination coefficients（R2） was higher than 0.999. When the spiked levels were 0.025, 0.050 and 0.25 mg/kg, the average recoveries of the three pesticides were between 95%-101%, with relative standard deviation（RSD） between 0.7% and2.2%（n=6）. The limits of quantitation（LOQ） for the three pesticides were 0.05, 0.05 and 0.2 μg/kg,respectively. This new method is accurate, sensitive, simple and suitable for the simultaneous determination of the above mentioned three pesticide residues in corn flour.

关 键 词：气相色谱-串联质谱 玉米粉 乙草胺 异丙甲草胺 三唑酮 残留 

分 类 号：S481.8[农业科学—农药学]