一起纽波特沙门菌引起的食物中毒检测及菌株同源性分析  

Determination,traceablility and homology analyis of a food poisoning case caused by Salmonella Newport

作　　者：金建潮[1] 曹春远[1] 何春荣[1] 林英华[1] 廖琳虹[1] 张景平[1,2] 陈前进[1,2] 

机构地区：[1]龙岩市疾病预防控制中心,福建龙岩364000 [2]福建医科大学龙岩市疾病预防控制中心教学点,福建龙岩364000

出　　处：《中国食品卫生杂志》2016年第2期172-174,共3页Chinese Journal of Food Hygiene

基　　金：福建省卫生厅青年科研课题项目(2014-1-96);2014年龙岩市第二批科技项目(2014LY29)

摘　　要：目的运用脉冲场凝胶电泳(PFGE)对一起食物中毒事件中分离的菌株进行同源性分析,为查明事件原因提供依据。方法采集患者、食品加工人员和剩余食物样本进行病原分离及鉴定,对分离到的菌株进行PFGE及耐药性分析。结果从采集的13份样本中检出4株纽波特沙门菌,其中1株来自病人样本,1株来自食品加工人员(厨师)样本,剩余牛肉、鸭肉各1株。4株菌株经PFGE分析,同源性为100%。4株分离菌株耐药谱相同。结论此次食物中毒由纽波特沙门菌引起,PFGE分型揭示菌株之间的流行病学联系,为事件的分析和溯源提供分子流行病学证据。Objective Using pulsed field gel electrophoresis for the separation of food poisoning strain for homology analysis,and providing a basis to find out the cause and traceability.Methods Samples from patients,food processing and leftovers were collected,isolated,identified and PFGE and antibiotic resistance analysis was performed.Results Four Salmonella Newport strains were detected in 13 samples,which were from the patient,the cooker,the remaining beef,and remaining duck.The PFGE patterns of the 4 strains were identical with the homology of 100%.4 isolates had to the same resistance spectrum.Conclusion The food poisoning was caused by Salmonella Newport.PFGE typing techniques revealed epidemiological link between the strains,providing evidence for molecular epidemiological analysis and traceability of events.

关 键 词：食物中毒 纽波特沙门菌 耐药性 脉冲场凝胶电泳 分型 食源性致病菌 食品安全 

分 类 号：R155.3[医药卫生—营养与食品卫生学]