Protective effects of astragaloside in rats with adriamycin nephropathy and underlying mechanism  

作　　者：WANG Na WEI Ri-Bao LI Qing-Ping YANG Xi CHEN Xiang-Mei 

机构地区：[1]Department of Nephrology,Chinese PLA General Hospital Chinese PLA Institute of Nephrology,State Key Laboratory of Kidney Diseases,National Clinical Research Center for Kidney Diseases,Beijing,China 100853

出　　处：《Chinese Journal of Natural Medicines》2016年第4期270-277,共8页中国天然药物（英文版）

基　　金：supported by the National Sciences Foundation of China(Nos.81273968 and 81471027);Ministerial projects of the National Working Commission on Aging(QLB2014W002);the“Four Hundreds Project of 301 Hospital(YS201408)

摘　　要：The present study was designed to determine the mechanism underlying the treatment of nephrotic syndrome using astragaloside by observing the effects of astragaloside on the expression of nephrin and podocin proteins and genes in kidneys of rats with adriamycin nephropathy. The rats were injected with adriamycin and, after successful model establishment, randomly divided into a model group, a Methylprednisolone(MP) group, and an astragaloside group. The 24-h complete urine samples were collected. Biochemical indicators were monitored, and kidney tissues were collected for pathological analysis using light microscopy and electron microscopy. The m RNA expression of nephrin and podocin was measured in the kidney tissues using the real-time q PCR, and the protein expression levels of nephrin and podocin were detected using Western blot analysis. At the end of 12 weeks of drug intervention, the urinary protein level was lower in the MP and astragaloside groups than that in the model group(P = 0.008 and P = 0.01, respectively). Serum albumin was higher in the MP and astragaloside groups than in the model group(P < 0.001 and P = 0.012, respectively). Podocytes in the MP group were nearly normal, and fusion of podocytes in the astragaloside group was significantly less than that in the control group. The nephrin and podocin m RNA and protein expression levels in the intervention groups were higher(P < 0.05) than that in the model group. Due to the increased expression of podocyte-related nephrin and podocin proteins, astragaloside maintained slit diaphragm integrity and decreased the level of proteinuria in rats with adriamycin nephropathy.The present study was designed to determine the mechanism underlying the treatment of nephrotic syndrome using astragaloside by observing the effects of astragaloside on the expression of nephrin and podocin proteins and genes in kidneys of rats with adriamycin nephropathy. The rats were injected with adriamycin and, after successful model establishment, randomly divided into a model group, a Methylprednisolone （MP） group, and an astragaloside group. The 24-h complete urine samples were collected. Biochemical indicators were monitored, and kidney tissues were collected for pathological analysis using light microscopy and electron microscopy. The mRNA expression of nephrin and podocin was measured in the kidney tissues using the real-time qPCR, and the protein expression levels of nephrin and podocin were detected using Western blot analysis. At the end of 12 weeks of drug intervention, the urinary protein level was lower in the MP and astragaloside groups than that in the model group （P = 0.008 and P = 0.01, respectively）. Serum albumin was higher in the MP and astragaloside groups than in the model group （P 〈 0.001 and P = 0.012, respectively）. Podocytes in the MP group were nearly normal, and fusion of podocytes in the astragaloside group was significantly less than that in the control group. The nephrin and podocin mRNA and protein expression levels in the intervention groups were higher （P 〈 0.05） than that in the model group. Due to the increased expression of podocyte-related nephrin and podocin proteins, astragaloside maintained slit diaphragm integrity and decreased the level of proteinuria in rats with adriamycin nephropathy.

关 键 词：ASTRAGALOSIDE Adriamycin nephropathy PODOCYTE NEPHRIN PODOCIN 

分 类 号：R965[医药卫生—药理学]