四倍体彩色马铃薯花青素含量及产量性状的QTL定位  

QTL location of anthocyanin content and yield in tetraploid pigmented potato

作　　者：崔阔澍[1] 于肖夏[1] 于卓[1] 姜超[1] 石悦[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《草业学报》2016年第5期116-124,共9页Acta Prataculturae Sinica

基　　金：内蒙古自然科学基金重大项目(2013ZD03);国家科技支撑计划项目(2012BAD02B05)资助

摘　　要：为确定彩色马铃薯薯块花青素含量、单株产量和商品薯率3个重要性状的QTL位点,以四倍体彩色马铃薯‘黑美人’בMIN-021’杂种F1代分离群体的210个单株无性株系及其亲本为材料,通过对这3个重要性状进行两年一点的观测试验,以及亲本间和杂种株系间的差异显著性分析,用TetraploidMap软件在已构建出的2张双亲的高密度彩色马铃薯分子遗传连锁图谱上分别定位其QTL。结果显示,这3个性状在亲本间和杂种株系间差异显著,且F_1群体单株株系间各性状观测值均呈正态分布,适合QTL分析。在母本‘黑美人’的遗传连锁图谱上检测到13个QTL,其中控制花青素含量的有5个、单株产量和商品薯率各有4个,遗传贡献率变幅为7.98%~19.62%。在父本‘MIN-021’的遗传连锁图谱上检测到11个QTL,其中花青素含量和单株产量各有4个、商品薯率有3个,遗传贡献率范围在8.70%~21.62%之间。To identify QTLs for three important traits, anthocyanin content, single plant yield and commodity rate in a pigmented potatoes, 210 plants from a F1 segregated population resulting from a cross of ＇heimeiren＇ × ＇MIN-021 ＇ and their parents, anthocyanin content, single plant yield and commodity potato rate were meas ured over two consecutive years. Differences between parents and the F1 population were assessed using vari ance analysis and the QTLs of each trait were constructed using high density genetic linkage maps with Tetra ploidMap software. The result demonstrated that the frequency distribution of all three traits was normal in the F1 population and therefore suitable for QTL analysis. A total of 13 QTLs were detected on a maternal linkage map of ＇heimeiren＇, including 5 QTLs controlling anthocyanin content, 4 QTLs controlling single plant yield and 4 QTLs controlling commodity potato rate. The genetic contribution ratio ranged from 7.98% to 19. 62%; a total of 11 QTLs were detected on a paternal linkage map of ＇MIN-021＇ , including 4 QTLs controlling antho cyanin content, 4 QTLs controlling single plant yield and 3 QTLs controlling commodity potato rate. The ge netic contribution ratio ranged from 8.70% to 21.62%.

关 键 词：彩色马铃薯 杂种F1分离群体 花青素含量 单株产量 商品薯率 QTL定位 

分 类 号：S532[农业科学—作物学]