响应面试验优化泡沫分离黄姜中薯蓣皂苷工艺  

Optimization of Foam Separation of Dioscin from Dioscorea zingiberensis C.H.Wright by Response Surface Methodology

作　　者：高中超[1] 张炜[1] 陈元涛[1] 刘海彬[1] 刘龙[1] 雷蕾[1] 

机构地区：[1]青海师范大学化学系,青海西宁810008

出　　处：《食品科学》2016年第8期26-31,共6页Food Science

基　　金：科技部科技人员服务企业项目(2009GJG20039)

摘　　要：在酶解黄姜的基础上,对黄姜薯蓣皂苷进行超声醇提,进而建立泡沫分离黄姜薯蓣皂苷的新方法。通过单因素试验及响应面试验设计,探讨稀释倍数、温度、装液量对泡沫法分离黄姜中薯蓣皂苷回收率及富集比的影响。结果得出,泡沫分离最佳条件为稀释倍数84、温度20℃、装液量436 m L,此条件下薯蓣皂苷的回收率为91.08%,富集比为7.53。研究表明,泡沫分离法是一种有效、简便、易于工业化生产和环境友好的黄姜薯蓣皂苷的分离富集方法。A novel method for recovering dioscin produced from the rhizomes of Dioscorea zingiberensis C.H.Wright by enzymatic hydrolysis and ultrasonic extraction with aqueous ethanol as the solvent was established by foam separation in this study.The effect of dilution factor,temperature and filling volume in the separation column on the recovery and enrichment ratio of dioscin was addressed using combination of one-factor-at-a-time method and response surface methodology.A dilution factor of 84,an operating temperature of 20 ℃ and filling 436 m L of sample into the separation column provided optimal conditions for separation of dioscin.Under these conditions,the maximum enrichment ratio and recovery of dioscin of 7.53 and 91.08% respectively were obtained.The procedure presented in this study is an effective,simple and environmentally compatible method thathas a great potential to be industrialized to separate dioscin from Dioscorea zingiberensis C.H.Wright using foam separation.

关 键 词：黄姜 薯蓣皂苷 泡沫分离法 响应面分析法 

分 类 号：O629.9[理学—有机化学]