兔皮胶原蛋白的提取及其结构鉴定  

Optimization of extraction process for rabbit-skin collagen and identification of its structure

作　　者：王雪蒙 于玮[1] 马良[1,2] 李洪军[1,2] 贺稚非[1,2] 张宇昊[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]西南大学国家食品科学与工程实验教学中心,重庆400715

出　　处：《食品与发酵工业》2016年第4期209-213,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(No.31301425);中央高校基本科研业务费重大项目(No.XDJK2015A015);中央高校基本科研业务费团队项目(No.2362014xk11);中国博士后科学基金面上项目(No.2014M562267);中国博士后科学基金特别资助项目(No.2015T80951);第四批重庆市高等学校优秀人才支持计划

摘　　要：为增加兔产业产品附加值,开发新型原料胶原蛋白产品,以兔皮为原料,采用酶法提取胶原蛋白。以胶原蛋白提取率为指标,研究胃蛋白酶用量、酶解液p H和酶解时间3个因素对胶原蛋白提取工艺的影响,确定胶原蛋白较优提取条件。单因素试验和正交试验结果表明,较优条件为酶解液p H值为1.9,酶解时间为4 h,胃蛋白酶用量为1%(w/w),在此条件下,胶原蛋白的提取率为(94.17±0.57)%,纯度为(86.12±0.15)%。聚丙烯酰氨凝胶电泳结果表明,兔皮胶原蛋白主要由α,β和γ3种亚基组分组成,为Ⅰ型胶原蛋白;差示量热扫描结果表明该胶原蛋白的热变性温度为44.59℃,热焓值为1.386 8 J/g,具有较高热稳定性。In order to increase rabbit industry value-added products and develop collagen products of new materials,collagen in rabbit skin was extracted with pepsin. With the collagen extraction rate as the evaluation indicator,the influence of enzyme solution p H、enzymolysis time and the dosage of pepsin to the extraction process of collagen were studied to determine the optimal extraction conditions. The single factor experiment and the orthogonal test results showed that the best processing combination was the enzyme solution pH 1. 9,enzymolysis time 4h and pepsin dosage 1%. Under this condition,the extraction rate of collagen was 94. 17% ± 0. 57%,with the collagen purity of86. 12% ± 0. 15%. The result of polyacrylamide gel electrophoresis showed that the subunits structure of collagen extracted from rabbit-skin was mainly of α、β and γ,which is type 1 collagen. The result of Scanning Differential Calorimeter analysis showed that the thermal denaturation temperature and enthalpy value of collagen was 44. 59 ℃ and1. 386 8 J / g,which proves that the collagen has a higher thermal stability.

关 键 词：兔皮 胶原蛋白 酶法 工艺优化 结构鉴定 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]