黑小豆种皮花色苷的提取及体外抗氧化活性研究  

Extraction and in vitro Antioxidant Activity of Anthocyanins Pigment from Vigna angularis(Willd.) Ohwi et Ohashi Hulls

作　　者：李芳[1] 阮长青[1] 汤凯欣 李巍威 张雪路 孙记涛 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《天然产物研究与开发》2016年第4期561-567,共7页Natural Product Research and Development

摘　　要：为了探索新的花色苷资源,以黑小豆种皮为原料,对其花色苷类色素的提取工艺进行了研究。通过单因素和L_9(3~4)正交试验,考察了乙醇浓度、料液比、温度和p H对粗提液中花色苷含量的影响。结果表明,最佳提取条件为:乙醇浓度60%、料液比1∶20(g∶m L)、温度50℃、p H=2.0。此条件下,黑小豆种皮粗提液中花色苷的含量最大(5.912 mg/g);黑小豆种皮花色苷粗提物得率为19.1%,纯度为3.06%;粗提物具有一定的总抗氧化能力和清除O^(-·)_2、·OH和DPPH自由基的能力。黑小豆种皮可作为一种新型花色苷资源加以利用。In order to explore new anthocyanins resources,extraction process for anthocyanin pigments from Vigna angularis（ Willd.） Ohwi et Ohashi hulls was developed and optimized by single factor and L9（ 3^4） orthogonal experiments.The optimal conditions for the extraction of anthocyanins from V. angularis hulls was 60% ethanol as extraction solvent,solid-liquid ratio of 1∶ 20（ g∶ m L）,temperature of 40 ℃ and p H 2. 0. Under these extraction conditions,the highest extraction amount for anthocyanins was 5. 912 mg / g with extraction yield of 19. 1% and purity of 3. 06%. V. angularis hulls crude extract showed a strong O2^-·,·OH and DPPH radical scavenging ability. V. hulls can be utilized as a new type of anthocyanins resource.

关 键 词：黑小豆种皮 花色苷 提取 抗氧化活性 

分 类 号：Q946.83[生物学—植物学]