牙龈卟啉单胞菌及其牙龈蛋白酶K对青少年牙龈健康的影响  

Influence of Porphyromonas gingivalis and its gingipain K in healthy gingiva of teenagers

作　　者：韩志强[1] 柏扬[2] 肖水清[1] 孙菲[3] 何萍[1] 

机构地区：[1]济南市口腔医院,济南250001 [2]济南市第一人民医院口腔科,济南250000 [3]滨州医学院口腔医学院正畸教研室,滨州256603

出　　处：《国际口腔医学杂志》2016年第3期283-287,共5页International Journal of Stomatology

摘　　要：目的分析牙龈卟啉单胞菌（P.gingivalis）及牙龈蛋白酶K（Kgp）对青少年牙龈健康的影响。方法收集12-17岁青少年牙龈正常组（50例）、牙龈炎症指数Ⅰ级组（25例）和牙龈炎症指数Ⅱ级组（32例）的3组龈沟液标本,应用16SrDNA PCR技术检测各样本中的P.gingivalis及Kgp。3组P.gingivalis和Kgp阳性检出率的比较采用χ2检验;P.gingivalis和Kgp的相对含量与牙龈炎症指数之间的关系采用Spearman相关分析。结果 P.gingivalis在牙龈炎症指数Ⅰ级组的检出率大于牙龈正常组,牙龈炎症指数Ⅱ级组的检出率大于牙龈炎症指数Ⅰ级组和牙龈正常组,牙龈炎症指数Ⅰ级组、Ⅱ级组与牙龈正常组间有明显差异（P〈0.01）,牙龈炎症指数Ⅰ级组与Ⅱ级组间差异具有统计学意义（P〈0.05）。Kgp在牙龈炎症指数Ⅰ级组的检出率大于牙龈正常组,牙龈炎症指数Ⅱ级组的检出率大于牙龈炎症指数Ⅰ级组和牙龈正常组,牙龈炎症指数Ⅰ级组与牙龈正常组间差异具有统计学意义（P〈0.05）,牙龈炎症指数Ⅱ级组与牙龈正常组间有明显差异（P〈0.01）。P.gingivalis和Kgp的检出率随着牙龈炎症指数的增加而升高。结论 P.gingivalis和Kgp与青少年牙龈健康密切相关。Objective To explore the relationship of Porphyromonas gingivalis（P. gingivalis）, gingipain K（Kgp） and puberty gingivitis in the subgingival. Methods Subjects（12- to 17-year-old children） included 50, 25, and 32 periodontally healthy, puberty gingivitis indexⅠ, and puberty gingivitis index Ⅱ children, respectively. 16 S rD NA PCR was conducted to detect P. gingivalis and Kgp from the samples. Chi-square test was performed to compare the prevalence of Kgp and P. gingivalis in the three groups. Spearman correlation analysis was also employed to evaluate the relationship between the relative quantity of Kgp and periodontal parameters. Results P. gingivalis in the puberty gingivitis index Ⅱ group was higher than that in the periodontally healthy group. Puberty gingivitis index Ⅱ was higher than puberty gingivitis index Ⅰ and periodontally healthy groups. Puberty gingivitis index Ⅱ, puberty gingivitis index Ⅱ, and periodontally healthy groups were significantly different from one another（P〈0.01）. Puberty gingivitis index Ⅰ was also significantly different from puberty gingivitis index Ⅱ（P〈0.05）. Kgp in the puberty gingivitis index Ⅰ group is higher than that in the periodontally healthy group（P〈0.01）. Puberty gingivitis index Ⅱ is higher than puberty gingivitis index Ⅰ and periodontally healthy groups. Puberty gingivitis index Ⅰ（P〈0.05） and puberty gingivitis index Ⅱ（P〈0.01） are signif icantly different from the periodontally healthy group. P. gingivalis and Kgp detection rates increased in the gingivitis index. Conclusion Kgp and P. gingivalis areclosely related to puberty gingivitis in the subgingival.

关 键 词：青少年 牙龈卟啉单胞菌 牙龈蛋白酶K 牙龈炎 

分 类 号：R780.2[医药卫生—口腔医学]