基因芯片筛选稳定转染FATE/BJ-HCC-2基因的肝癌细胞中肿瘤转移相关差异表达基因  

Screening of tumor metastasis related differentially expressed genes in hepatocellular carcinoma cells with FATE/BJ-HCC-2 gene stable transfection by gene chip

作　　者：葛丽丽[1] 杨小昂[2] 朴军颜[2] 尹艳慧[3] 张毓[3] 

机构地区：[1]河南省郑州市儿童医院检验科,450053 [2]郑州大学医药科学研究院,450052 [3]北京大学免疫学系T细胞室,100083

出　　处：《重庆医学》2016年第12期1595-1597,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81071724);河南省基础与前沿基金资助项目(142300413224)

摘　　要：目的筛选稳定转染FATE/BJ-HCC-2基因的肝癌细胞中肿瘤转移相关的差异表达基因。方法分别提取稳定转染FATE/BJ-HCC-2的肝癌细胞(5B4)及转染空质粒的对照细胞(Mock)总RNA,通过基因芯片技术筛选差异表达基因。结果与Mock细胞比较,5B4细胞共筛选出1 694个差异表达基因,有11个基因表达差异明显,其中MMP-1、PTGS2、FN、CA9、IL-8、ILK、Areg 7个基因在转染FATE/BJ-HCC-2基因后表达量明显上升,差异倍数分别为81.80、49.86、11.30、16.26,3.48、2.79、2.20。E-cadherin、RhoBTB3、ALPP、HLA-DRB 4个基因表达量明显下降,差异倍数分别为-5.42、-2.23、-5.93、-8.03。结论采用基因芯片技术对FATE/BJ-HCC-2参与的肝癌转移的差异表达基因进行细致的筛选,其最终目的是为研究肝癌转移机制打下坚实的理论基础。Objective To screen the tumor metastasis related differentially expressed genes in hepatocelluar carcinoma（HCC）cells 7402 after stable transfection with FATE/BJ-HCC-2gene.Methods Total RNA was extracted from FATE/BJ–HCC-2-transfected HCC（5B4）cells and empty vector control（Mock）cells respectively.Differentially expressed genes were obtained using cDNA microarray.Results Compared with Mock cells,a total of 1 694 differentially expressed genes were screened out in5B4 cells,the 11 gene expressions had obvious differences,among which the expression amounts in 7genes were significantly increased,including MMP-1,PTGS2,FN,CA9,IL-8,ILK and Areg.The fold changes were 81.80,49.86,11.30,16.26,3.48,2.79 and 2.20,respectively.The expression amounts in 4 genes were significantly decreased,including E-cadherin,E-cadherin,RHOBTB3,ALPP and HLA-DRB4.The fold changes were-5.42,-2.23,-5.93and-8.03,respectively.Conclusion Adopting gene microarray technology can carefully screen the differentially expressed genes of FATE/BJ-HCC-2involved HCC metastasis,its final goal is to lay a solid theoretical foundation for studying the HCC metastasis mechanism.

关 键 词：肝肿瘤 基因芯片 基因差异表达 

分 类 号：R735.7[医药卫生—肿瘤]