降解玉米赤霉烯酮芽孢杆菌的筛选和鉴定  

Screening and identification of degradation zearalenone Bacillus

作　　者：葛婵婵 熊犍[1] 赵晨[2] 申琳[3] 张晓琳[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510641 [2]国家粮食局科学研究院,北京100037 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《粮油食品科技》2016年第3期86-91,共6页Science and Technology of Cereals,Oils and Foods

基　　金：国家自然科学基金(31302007);"十二五"农村领域国家科技计划课题(2011BAD26B002-5)

摘　　要：从400株芽孢菌中筛选到对玉米赤霉烯酮(ZEN)有较好清除作用的两株芽孢杆菌373-2和411-1,分别与玉米赤霉烯酮(15μg/m L)共培养8 h和6 h后,将其完全清除。通过16S rDNA序列分析鉴定,373-2和411-1分别为一株新种芽孢杆菌和枯草芽孢杆菌(Bacillus subtilis)。对两株菌的玉米赤霉烯酮清除机理进行初步分析,结果表明,清除作用主要源自菌株胞外酶的降解活性。Two Bacillus strains 373- 2 and 411- 1 showing good performance in zearalenone elimination,were screened from 400 strains of Bacillus. Zearalenone( 15 μg / m L) could be eliminated completely after co- culture with the screened strains for 8 and 6 hours,respectively. Strain 373- 2 and 411- 1 were identified as a new species of Bacillus and Bacillus subtilis based on 16 S r DNA sequence analysis. The mechanism of zearalenone elimination was also preliminarily investigated,and the result demonstrated that the main activity of zearalenone scavenging was due to the extracellular enzyme.

关 键 词：玉米赤霉烯酮 芽孢杆菌 筛选 降解 

分 类 号：TS201.3[轻工技术与工程—食品科学]