微波辅助萃取马兰头抑制黑曲霉活性成分的工艺优化  

Optimization of Microwave-assisted Extraction Process for Bioactive Compounds of Aspergillus niger Inhibitors from Kalimeris indica L.by Orthogonal Design

作　　者：马佳凤 曾秀桃 朱晨[1] 林颖[1] 王慧敏[1] 徐丽珊[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《食品研究与开发》2016年第6期77-80,共4页Food Research and Development

摘　　要：以马兰头(Kalimeris indica L.)为原料,在单因素试验的基础上,利用正交试验法优化微波辅助萃取马兰头抑制黑曲霉活性成分的工艺。结果表明,各因素对萃取马兰头抑制黑曲霉活性成分影响程度大小顺序为:料液比>温度>功率>时间,并得到最佳萃取工艺为功率700 W,温度70℃,时间3 min,料液比1∶70(g/m L),验证表明试验结果真实可信。采用最佳萃取工艺制得马兰头抑制黑曲霉活性成分粗品的产率为44.62%,进一步试验结果表明该提取物对黑曲霉(Aspergillus niger)、青霉(Penicillium)均有较好的抑制效果,其对黑曲霉、青霉的MIC均约为0.94 mg/m L,表明马兰头提取物具有抑制真菌生长的潜能。On the basis of single factor experiment,the microwave-assisted extraction technique of bioactive compounds of Aspergillus niger inhibitors from Kalimeris indica L. was optimized by orthogonal design. The results showed that the primary and secondary order of influence of the factors microwave-assisted extraction method for bioactive compounds of Aspergillus niger inhibitors from Kalimeris indica L. was confirmed：the ratio of solid to liquid 〉temperature 〉power 〉time. The optimum process condition was confirmed that the yields of crude extract was 44.62 % could be obtained when extraction power was 700 W,extraction temperature was70 ℃,extraction time was 3 min,the ratio of solid to liquid was 1 ∶ 70（g/m L）. Verification showed the result was authentic. Further experimental results showed that the extract had better inhibitory activity on Aspergillus niger and Penicillium. The Minimum Inhibition Concentration（MIC）for Aspergillus niger and Penicillium are both about 0.94 mg/m L,showed the extract of Kalimeris indica L. has the potential to inhibit fungal growth.

关 键 词：马兰头 抑制黑曲霉活性 微波辅助萃取 正交试验法 

分 类 号：TQ914.1[化学工程]