黑曲霉高产β-葡萄糖苷酶菌株的诱变、筛选及发酵条件优化  

Mutagenesis, Screening and Optimization of Fermentation Conditions on Aspergillus niger High-yield β-glucosidase Neuraminidase Strain

作　　者：陈娜 付传颖 张玉新[3] 贾文娣[4] 

机构地区：[1]唐山市农业科学院,唐山063000 [2]唐山师范学院,唐山063000 [3]唐山市动物卫生监督所,唐山063000 [4]中国科学院天津工业生物技术研究所,天津300308

出　　处：《基因组学与应用生物学》2016年第4期901-906,共6页Genomics and Applied Biology

基　　金：国家高技术研究发展计划(863计划)No.2012AA023202项目资助

摘　　要：以黑曲霉TJ02为出发菌,对其孢子进行硫酸二乙酯化学诱变,筛选得到遗传稳定的β-葡萄糖苷酶高产菌株DES-7。诱变株DES-7产酶能力可达28 IU/mL以上,较出发菌株提高30%。同时,对该菌株的发酵培养基进行优化,以玉米芯为碳源,酵母粉和硫酸铵为氮源,其产酶能力达到39 IU/mL,较优化前提高了39.3%。此外,对该菌株的β-葡萄糖苷酶的最适温度和pH以及温度稳定性和pH稳定性进行了测定。Based on the strain of Aspergillus niger TJ02, a β-glucosidase high-yield mutant DES-7 with genetic stability was obtained by diethyl sulfate mutagenisis. Compared with the original strain, the enzyme yield of strain DES-7 up to 28 IU/mL with 30% increasement. Meanwhile, the components of fermentation medium were optimized and the enzyme production yield reached 39 IU/mL with 39.3% increasement, taking corncob and yeast powder and ammonium sulfate as carbon source and nitrogen source, respectively. Besides, the optimal temperature and pH, the stability of temperature/pH of the β-glucosidase were also determined.

关 键 词：黑曲霉 Β-葡萄糖苷酶 诱变 碳氮源 

分 类 号：Q93[生物学—微生物学] TQ925[轻工技术与工程—发酵工程]