黑木耳多糖对内毒素诱导急性肾脏损伤的保护作用及机制研究  

Auricularia Auricular-judae Polysaccharide Attenuates Lipopolysaccharide-induced Acute Kidney Injury by Inhibiting Oxidative Stress

作　　者：刘斌[1] 哈尼再尔.热合曼 高阳[1] 李转运[1] 冉新建[2] 罗晶晶[2] 孙湛[2] 

机构地区：[1]新疆医科大学临床医学院,新疆乌鲁木齐830054 [2]新疆医科大学基础医学院,新疆乌鲁木齐830054

出　　处：《现代生物医学进展》2016年第13期2438-2441,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81260454);新疆维吾尔自治区大学生创新实验项目(CX2014021)

摘　　要：目的:探讨黑木耳多糖对内毒素(LPS)诱导大鼠急性肾损害的保护效应及可能机制。方法:健康雄性SD大鼠,分为正常对照组、LPS组和黑木耳多糖组(LPS+黑木耳多糖)。根据分组,分别于复制模型前7 d给予生理盐水或1%黑木耳多糖(15 m L/kg)预防性灌胃。第8 d腹腔注射生理盐水或LPS(8 mg/kg),造模12 h后,通过腹主动脉采血检测血清尿素氮(BUN)、肌酐(Cr)水平,取肾脏制备匀浆检测肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)水平。结果:用黑木耳多糖干预后,大鼠血清中BUN、Cr水平均显著低于LPS组(P<0.05);LPS组MDA含量较正常对照组增高,SOD、T-AOC水平降低,差异有统计学意义(P<0.05);黑木耳多糖组MDA含量较LPS组降低,SOD、T-AOC水平升高,差异有统计学意义(P<0.05)。结论:黑木耳多糖能显著提高内毒素血症大鼠的抗氧化能力,对肾脏组织有保护作用。Objective： To investigate the role ofAuricularia auricular-judae polysaccharide （AAP） in acute kidney injury induced by lipopolysaccharide （LPS） in rats and to further explore the possible underlying mechanisms. Methods： Adult Sprague-Dawley rats were randomly assigned into the control, LPS, and LPS plus AAP groups. Rats were injected with LPS （10 mg/kg, i.p.） to induce acute kidney injury. Rats in the LPS plus AAP group were treated with AAP for 7 days before the induction of ALI. Blood urea nitrogen（BUN） and creatinine （Cr） in serum were measured. Superoxide dismutase（SOD）, total antioxidant capacity （T-AOC）activity and malondialde- hyde （MDA） level in kidney were measured. Pathological changes of kidney tissues were observed by HE staining. Results： The data showed that treatment with AAP group significantly improved LPS-induced lung pathological changes, decreased the BUN and Cr level, and reduced MDA level, increased the SOD, T-AOC activity. Conclusions： The results indicated that AAP has a protective effect on LPS-induced acute kidney injury in rat.

关 键 词：黑木耳多糖 脂多糖 急性肾损伤 氧化应激 

分 类 号：R-33[医药卫生] R692